Moissenet D, Goujon C P, Garbarg-Chenon A, Vu-Thien H
Service de Microbiologie, Hôpital d'Enfants Armand-Trousseau, Paris, France.
J Clin Microbiol. 1999 Jun;37(6):1777-81. doi: 10.1128/JCM.37.6.1777-1781.1999.
CDC group IV c-2, an environmental gram-negative bacillus recently proposed for inclusion in the genus Ralstonia, has been isolated in several human infections. Biochemical characterization and 16S ribosomal DNA (rDNA) sequencing with phylogenetic analysis were used to characterize eight clinical isolates and four type strains. Other typing tools, such as pulsed-field gel electrophoresis (PFGE) and randomly amplified polymorphic DNA (RAPD) analysis, were also used. PFGE typing of clinical isolates was unsuccessful because the DNA was degraded, and RAPD analysis was poorly discriminatory. In contrast, the type strains were clearly distinguished with both PFGE and RAPD analysis. All of the 16S rDNA sequences were identical. Comparison of the 16S rDNA sequences to the GenBank sequences showed that they were consistent with CDC group IV c-2 belonging to the genus Ralstonia. The closest matches were obtained with Ralstonia eutropha. However, four differences in 32 biochemical tests separated R. eutropha from CDC group IV c-2, which suggests that CDC group IV c-2 is a new species of the genus Ralstonia.
疾病控制与预防中心（CDC）IV c-2群，一种最近提议归入罗尔斯通氏菌属的环境革兰氏阴性杆菌，已在数起人类感染病例中分离得到。采用生化特性鉴定以及16S核糖体DNA（rDNA）测序并进行系统发育分析，对8株临床分离株和4株模式菌株进行了特征鉴定。还使用了其他分型工具，如脉冲场凝胶电泳（PFGE）和随机扩增多态性DNA（RAPD）分析。由于DNA降解，临床分离株的PFGE分型未成功，RAPD分析的鉴别能力也较差。相比之下，模式菌株通过PFGE和RAPD分析都能清晰区分。所有16S rDNA序列均相同。将16S rDNA序列与GenBank序列进行比较，结果表明它们与属于罗尔斯通氏菌属的CDC IV c-2群一致。与真养罗尔斯通氏菌的匹配度最高。然而，在32项生化试验中有4项差异将真养罗尔斯通氏菌与CDC IV c-2群区分开来，这表明CDC IV c-2群是罗尔斯通氏菌属的一个新物种。
