蛙骨骼肌钠通道的离子选择性
Ionic selectivity of the sodium channel of frog skeletal muscle.
作者信息
Campbell D T
出版信息
J Gen Physiol. 1976 Mar;67(3):295-307. doi: 10.1085/jgp.67.3.295.
Abstract
The ionic selectivity of the Na channel to a variety of metal and organic cations is studied in frog semitendinosus muscle. Na channel currents are measured under voltage clamp conditions in fibers bathed in solutions with all Na+ replaced by a test ion. Permeability ratios are calculated from measured reversal potentials using the Goldman-Hodgkin-Katz equation. The permeability sequence was Na+ approximately Li+ approximately hydroxylammonium greater than hydrazinium greater than ammonium greater than guanidinium greater than K+ greater than aminoguanidinium in the ratios 1:0.96:0.94:0.31:0.11:0.093:0.048:0.031. No inward currents were observed for Ca++, methylammonium, methylguanidinium, tetraethylammonium, and tetramethylammonium. The results are consistent with the Hille model of the Na channel selectivity filter of the node of Ranvier and suggest that the selectivity filter of the two channels is the same.
摘要
在青蛙半腱肌中研究了钠通道对多种金属和有机阳离子的离子选择性。在电压钳制条件下，测量浸泡在所有Na⁺被测试离子取代的溶液中的纤维的钠通道电流。使用戈德曼-霍奇金- Katz方程根据测量的反转电位计算渗透率比。渗透率顺序为Na⁺≈Li⁺≈羟铵＞肼＞铵＞胍＞K⁺＞氨基胍，比例为1:0.96:0.94:0.31:0.11:0.093:0.048:0.031。对于Ca²⁺、甲铵、甲基胍、四乙铵和四甲铵未观察到内向电流。结果与郎飞结钠通道选择性过滤器的希勒模型一致，并表明这两种通道的选择性过滤器是相同的。
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