Salman Ahmad, Ramesh Jagannathan, Erukhimovitch Vitaly, Talyshinsky Marina, Mordechai Shaul, Huleihel Mahmoud
Department of Physics, Ben Gurion University of the Negev, 84105, Beer-Sheva, Israel.
J Biochem Biophys Methods. 2003 Feb 28;55(2):141-53. doi: 10.1016/s0165-022x(02)00182-3.
Fourier transform infrared microspectroscopy (FTIR-MSP), which is based on the characteristic molecular vibrational spectra of cells, was used to investigate spectral differences between normal primary rabbit bone marrow (BM) cells and bone marrow cells transformed (BMT) by murine sarcoma virus (MuSV). Primary cells, rather than cell lines, were used for this research because primary cells are similar to normal tissue cells in most of their characteristics. Our results showed dramatic changes in absorbance between the control cells and MuSV124-transformed cells. Various biological markers, such as the phosphate level and the RNA/DNA obtained, based on the analysis of the FTIR-MSP spectra, also displayed significant differences between the control and transformed cells. Preliminary results suggested that the cluster analysis performed on the FTIR-MSP spectra yielded 100% accuracy in classifying both types of cells.
傅里叶变换红外显微光谱法（FTIR-MSP）基于细胞的特征分子振动光谱，用于研究正常原代兔骨髓（BM）细胞与被鼠肉瘤病毒（MuSV）转化的骨髓细胞（BMT）之间的光谱差异。本研究使用的是原代细胞而非细胞系，因为原代细胞在大多数特征上与正常组织细胞相似。我们的结果显示，对照细胞和MuSV124转化细胞之间的吸光度有显著变化。基于FTIR-MSP光谱分析得到的各种生物标志物，如磷酸盐水平和RNA/DNA，在对照细胞和转化细胞之间也显示出显著差异。初步结果表明，对FTIR-MSP光谱进行的聚类分析在对两种类型的细胞进行分类时准确率达到100%。
