Mordechai S, Sahu R K, Hammody Z, Mark S, Kantarovich K, Guterman H, Podshyvalov A, Goldstein J, Argov S
Department of Physics, Ben Gurion University of the Negev, Beer Sheva, 84105, Israel.
J Microsc. 2004 Jul;215(Pt 1):86-91. doi: 10.1111/j.0022-2720.2004.01356.x.
Detection of malignancy at early stages is crucial in cancer prevention and management. Fourier transform infrared (FTIR) spectroscopy has shown promise as a non-invasive method with diagnostic potential in cancer detection. Studies were conducted with formalin-fixed biopsies of melanoma and cervical cancer by FTIR microspectroscopy (FTIR-MSP) to detect common biomarkers, which occurred in both types of cancer distinguishing them from the respective non-malignant tissues. Both types of cancer are diagnosed on skin surfaces. The spectra were analysed for changes in levels of biomolecules such as RNA, DNA, phosphates and carbohydrate (glycogen). Whereas carbohydrate levels showed a good diagnostic potential for detection of cervical cancer, this was not the case for melanoma. However, variation of the RNA/DNA ratio as measured at I(1121)/I(1020) showed similar trends between non-malignant and malignant tissues in both types of cancer. The ratio was higher for malignant tissues in both types of cancer.
癌症早期恶性肿瘤的检测对于癌症预防和管理至关重要。傅里叶变换红外（FTIR）光谱已显示出作为一种在癌症检测中具有诊断潜力的非侵入性方法的前景。通过傅里叶变换红外显微光谱（FTIR-MSP）对黑色素瘤和宫颈癌的福尔马林固定活检组织进行了研究，以检测在这两种癌症中均出现的常见生物标志物，这些生物标志物将它们与各自的非恶性组织区分开来。这两种癌症均在皮肤表面进行诊断。对光谱分析了诸如RNA、DNA、磷酸盐和碳水化合物（糖原）等生物分子水平的变化。虽然碳水化合物水平对宫颈癌检测具有良好的诊断潜力，但黑色素瘤并非如此。然而，在I(1121)/I(1020)处测量的RNA/DNA比值变化在两种癌症的非恶性和恶性组织之间显示出相似趋势。两种癌症的恶性组织中该比值均较高。
