Sheremet'ev Iu A, Sheremet'eva A V
State Agricultural Academy, pr. Gagarina 97, Nizhnii Novgorod, 603107 Russia.
Biofizika. 2003 Jan-Feb;48(1):63-7.
It was shown that the treatment of erythrocytes with low concentrations of glutaraldehyde (0.01-0.1%) for 30-120 min to a variable extent the aggregation induced by 40-330 microM La3+. The effect of glutaraldehyde on the aggregation increased with concentration and time of fixation. La3+ ions decreased to a similar extent the electrophoretic mobility of intact erythrocytes and erythrocytes treated with 0.1% glutaraldehyde. No relationship was found between the change in the negative charge on the erythrocytes and the degree of their aggregation. Neuraminidase and trypsin were shown to decrease the surface charge on the erythrocytes and the aggregation of fixed erythrocytes.
结果表明，用低浓度戊二醛（0.01 - 0.1%）处理红细胞30 - 120分钟，可在不同程度上抑制40 - 330微摩尔La3+诱导的聚集。戊二醛对聚集的影响随固定浓度和时间的增加而增强。La3+离子使完整红细胞和用0.1%戊二醛处理的红细胞的电泳迁移率降低程度相似。红细胞负电荷的变化与其聚集程度之间未发现相关性。神经氨酸酶和胰蛋白酶可降低红细胞表面电荷以及固定红细胞的聚集。
