Knott Andrew W, Erwin Christopher R, Profitt Sherri A, Juno Russell J, Warner Brad W
Division of Pediatric Surgery, Cincinnati Children's Hospital Medical Center, and the Department of Surgery, University of Cincinnati College of Medicine, Cincinnati, Ohio 45229-3039, USA.
J Pediatr Surg. 2003 Mar;38(3):440-5. doi: 10.1053/jpsu.2003.50076.
BACKGROUND/PURPOSE: Epidermal growth factor (EGF) and its receptor (EGFR) are key components in the genesis of adaptation after small bowel resection (SBR). Within intestinal homogenates, EGFR expression is increased after SBR; however, the exact cells responsible for altered EGFR expression are unknown. In this study, laser capture microdissection (LCM) microscopy was used to elucidate the specific cellular compartment(s) responsible for postresection changes in EGFR expression.
Male ICR mice underwent a 50% proximal SBR or sham operation. After 3 days, frozen sections were taken from the remnant ileum. Individual cells from villi, crypt, muscularis, and mesenchymal compartments were isolated by LCM. EGFR mRNA expression for each cell compartment was quantified using real-time polymerase chain reaction (PCR).
EGFR expression was increased after SBR within the crypt (2-fold) and muscularis compartments (3-fold). There were no changes detected after SBR in the villus tips or mesenchymal compartments.
Increased expression of EGFR in crypts directly correlates with the zone of cell proliferation and supports the hypothesis that EGFR signaling is crucial for the mitogenic stimulus for adaptation. The finding of increased EGFR expression in the muscular compartment is novel and may implicate a role for EGFR as a mediator of the muscular hyperplasia seen after massive SBR.
背景/目的：表皮生长因子（EGF）及其受体（EGFR）是小肠切除术后（SBR）适应性形成过程中的关键成分。在肠匀浆中，SBR后EGFR表达增加；然而，导致EGFR表达改变的确切细胞尚不清楚。在本研究中，使用激光捕获显微切割（LCM）显微镜来阐明负责切除后EGFR表达变化的特定细胞区室。
雄性ICR小鼠接受50%近端SBR或假手术。3天后，从残余回肠获取冰冻切片。通过LCM从绒毛、隐窝、肌层和间充质区室中分离出单个细胞。使用实时聚合酶链反应（PCR）对每个细胞区室的EGFR mRNA表达进行定量。
SBR后，隐窝（2倍）和肌层区室（3倍）中的EGFR表达增加。SBR后在绒毛顶端或间充质区室未检测到变化。
隐窝中EGFR表达的增加与细胞增殖区域直接相关，并支持EGFR信号传导对于适应性促有丝分裂刺激至关重要的假说。在肌层区室中EGFR表达增加这一发现是新颖的，可能意味着EGFR在大规模SBR后所见的肌肉增生中起介导作用。
