从绵羊呼吸道分离出的糖酵解支原体的比较与特性分析

The comparison and characterisation of glycolytic mycoplasmas isolated from the respiratory tract of sheep.

作者信息

Jones G E, Foggie A, Mould D L, Livitt S

出版信息

J Med Microbiol. 1976 Feb;9(1):39-52. doi: 10.1099/00222615-9-1-39.

Abstract

Nine strains of glycolytic mycoplasmas isolated from the respiratory tract of apparently healthy sheep, pneumonic sheep and sheep with pulmonary adenomatosis (SPA) were compared with a Queensland strain (Y98) of Mycoplasma ovipneumoniae. All strains were very similar in their reactions in 14 biochemical tests and in their sensitivities to optochin, digitonin, sodium polyanethol sulphonate, and 11 antibiotics. Polyacrylamide-gel electrophoresis and serological cross-reactions by the agar-gel double diffusion, metabolic inhibition (MI) and growht-inhibition (GI) tests also showed that all strains could be classified as M. ovipneumoniae. The MI and GI tests, however, showed considerable intraspecific differences among strains, with apparent polarisation of SPA strains and non-SPA strains at opposite ends of the antigenic spectrum. Two representative strains were tested by the MI test against antisera to 39 mycoplasma species or serogroups, with negative results.

摘要

将从表面健康的绵羊、患肺炎的绵羊以及患有肺腺瘤病（SPA）的绵羊呼吸道中分离出的9株糖酵解支原体菌株与绵羊肺炎支原体的昆士兰菌株（Y98）进行了比较。在14项生化试验中的反应以及对奥普托欣、洋地黄皂苷、聚茴香脑磺酸钠和11种抗生素的敏感性方面，所有菌株都非常相似。聚丙烯酰胺凝胶电泳以及琼脂凝胶双向扩散、代谢抑制（MI）和生长抑制（GI）试验的血清学交叉反应也表明，所有菌株都可归类为绵羊肺炎支原体。然而，MI和GI试验显示菌株之间存在相当大的种内差异，SPA菌株和非SPA菌株在抗原谱的两端明显极化。通过MI试验，用针对39种支原体物种或血清群的抗血清对两株代表性菌株进行了检测，结果均为阴性。