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大鼠胆管癌发生过程中转化生长因子β、转化生长因子β受体II mRNA及端粒酶活性的原位检测

In situ detection of TGF betas, TGF beta receptor II mRNA and telomerase activity in rat cholangiocarcinogenesis.

作者信息

Lu Jian-Ping, Mao Jian-Qun, Li Ming-Sheng, Lu Shi-Lun, Hu Xi-Qi, Zhu Shi-Neng, Nomura Shintaro

机构信息

Department of Pathology, Medical Center of Fudan University (Former Shanghai Medical University), Shanghai 200032, China.

出版信息

World J Gastroenterol. 2003 Mar;9(3):590-4. doi: 10.3748/wjg.v9.i3.590.

DOI:10.3748/wjg.v9.i3.590
PMID:12632524
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4621588/
Abstract

AIM

Initial report on the in situ examination of the mRNA expression of transforming growth factor betas (TGFbetas), TGFbeta type II receptor (TbetaRII) and telomerase activity in the experimental rat liver tissue during cholangiocarcinogenesis.

METHODS

Rat liver cholangiocarcinogenesis was induced by 3'-methyl 4-dimethylazobenzene (3'Me-DAB). In situ hybridization was used to examine the TGFbetas) and TGFbeta type II receptor (TbetaRII) mRNA, in situ TRAP was used to check the telomerase activity in the tissue samples.

RESULTS

There was no TGFbetas, TbetaRII mRNA expression or telomerase activity in the control rat cholangiocytes. The expression of TGFbeta1, TbetaRII was increased in regenerative, hyperplastic, dysplastic cholangiocytes and cholangiocarcinoma (CC) cells. The expression of TGFbeta2 mRNA was observed in only a part of hyperplastic, dysplastic cholangiocytes. TGFbeta3 expression was very weak, only in hyperplastic lesion. There was positive telomerase activity in the regenerative, hyperplastic, dysplastic cholangiocytes, and CC cells. Stroma fibroblasts of these lesions also showed positive TGFbetas, TbetaRII mRNA expression and telomerase activity.

CONCLUSION

There were TGFbetas, TbetaRII expression and telomerase activity in hyperplastic, dysplastic cholangiocytes, cholangiocarcinoma cells as well as in stroma fibroblasts during cholangiocarcinogenesis. Their expression or activity is important in cholangiocarcinogenesis andstroma formation.

摘要

目的

关于在胆管癌发生过程中对实验大鼠肝组织中转化生长因子β（TGFβ）、Ⅱ型TGFβ受体（TβRⅡ）的mRNA表达及端粒酶活性进行原位检测的初步报告。

方法

用3'-甲基-4-二甲基偶氮苯（3'Me-DAB）诱导大鼠肝胆管癌发生。采用原位杂交检测TGFβ和Ⅱ型TGFβ受体（TβRⅡ）的mRNA，用原位TRAP检测组织样本中的端粒酶活性。

结果

对照大鼠胆管细胞中未检测到TGFβ、TβRⅡ mRNA表达或端粒酶活性。TGFβ1、TβRⅡ在再生、增生、发育异常的胆管细胞及胆管癌细胞（CC）中的表达增加。仅在部分增生、发育异常的胆管细胞中观察到TGFβ2 mRNA表达。TGFβ3表达非常弱，仅在增生性病变中有表达。在再生、增生、发育异常的胆管细胞及CC细胞中存在端粒酶活性。这些病变的基质成纤维细胞也显示出TGFβ、TβRⅡ mRNA表达阳性及端粒酶活性。

结论

在胆管癌发生过程中，增生、发育异常的胆管细胞、胆管癌细胞以及基质成纤维细胞中存在TGFβ、TβRⅡ表达及端粒酶活性。它们的表达或活性在胆管癌发生及基质形成中具有重要意义。

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