Owen J D, Steggall M, Eyring E M
J Membr Biol. 1976 Mar 18;26(2-3):287-99. doi: 10.1007/BF01868878.
The reflection coefficient, sigma, for several small permeant nonelectrolytes was determined for dog and beef red blood cell membranes. Our sigma values were considerably higher than those previously reported for dog cells; e.g., out sigma urea was 87% higher than the sigma urea of Rich, Sha'afi, Barton and Solomon (J. Gen. Physiol. 50: 2391, 1967). Our sigma values for urea were only slightly greater in beef cells than previously reported by Farmer and Macey (Biochim. Biophys. Acta 290: 290, 1972). We found that a trend exists when (1 - sigma) is plotted against the log of the permeability coefficient, omega. This observation is also consistent with our previously reported sigma data for human red cell membranes (Owen & Eyring, J. Gen. Physiol. 66: 241, 1972). This trend suggests that small hydrophilic molecules interact highly with cell membrane water. The exceptions to this trend were lipophilic molecules, indicating they do not interact with water while penetrating the red cell membrane.
测定了几种小分子渗透性非电解质对狗和牛红细胞膜的反射系数σ。我们得到的σ值比先前报道的狗细胞的σ值高得多；例如，我们得到的尿素σ值比Rich、Sha'afi、Barton和Solomon（《普通生理学杂志》50: 2391, 1967）报道的尿素σ值高87%。我们得到的牛肉细胞尿素σ值仅比Farmer和Macey（《生物化学与生物物理学报》290: 290, 1972）先前报道的值略高。我们发现，当将(1 - σ) 对渗透系数ω的对数作图时，存在一种趋势。这一观察结果也与我们先前报道的人红细胞膜的σ数据一致（Owen和Eyring，《普通生理学杂志》66: 241, 1972）。这种趋势表明，小分子亲水性分子与细胞膜水有高度相互作用。这种趋势的例外情况是亲脂性分子，这表明它们在穿透红细胞膜时不与水相互作用。
