Okada H, Usuda H, Tajima T, Kawahara M, Yoshino T, Rikihisa Y
Department of Veterinary Pathology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, Hokkaido, 069-8501, Japan.
J Comp Pathol. 2003 Feb-Apr;128(2-3):182-7. doi: 10.1053/jcpa.2002.0624.
Specific identification of ehrlichiae in the tissues and determination of their distribution is difficult. In this study, an in-situ hybridization method was developed to detect ehrlichial 16S rRNA in tissue specimens from mice experimentally infected with the HF strain. This strain is closely related to Ehrlichia chaffeensis, the agent of human monocytic ehrlichiosis. HF strain-specific 16S rRNA was detected in endothelial cells and monocyte-macrophages in the liver, lungs, bone marrow, spleen, lymph nodes, and large and small intestinal tissues. The results suggest that the in-situ hybridization method with a digoxigenin-labelled RNA probe specific to ehrlichial 16S rRNA will be useful for post-mortem diagnosis and for the histopathological investigation of ehrlichial infection.
在组织中特异性鉴定埃立克体及其分布情况颇具难度。在本研究中，开发了一种原位杂交方法，用于检测实验感染HF株的小鼠组织标本中的埃立克体16S rRNA。该菌株与引起人类单核细胞埃立克体病的查菲埃立克体密切相关。在肝脏、肺、骨髓、脾脏、淋巴结以及大小肠组织的内皮细胞和单核巨噬细胞中检测到了HF株特异性16S rRNA。结果表明，使用地高辛标记的针对埃立克体16S rRNA的RNA探针的原位杂交方法，将有助于死后诊断以及埃立克体感染的组织病理学研究。
