van Furth R, Fedorko M E
Lab Invest. 1976 Apr;34(4):440-50.
Recently a new method was developed to culture bone marrow cells in a liquid medium on a glass surface. Two kinds of colonies develop in these cultures, namely mononuclear phagocyte and granulocyte colonies. The study of the ultrastructure of the cells in the mononuclear phagocyte colonies was the primary aim of the present study. The architecture of the mononuclear phagocyte colonies appeared to be quite different from that of the granulocyte colonies, since in the latter, the cells lie close together in dense clusters, whereas in mononuclear phagocyte colonies the cells are more loosely dispersed with the highest cell density at the center and stellate orientation of the cells at the periphery. However, both kind of colonies grow entirely separate from each other and mixed colonies are not observed. Electron microscopy showed that there are three types of cell in the mononuclear phagocyte colonies, i.e., monoblasts, promonocytes, and macrophages. The ultrastructure of the promonocytes and macrophages of the colonies is similar to that of the same types of cell isolated directly from the mouse. The monoblast, the most immature cell of mononuclear phagocyte colonies has not been characterized before. The ultrastructure of this cell is clearly distinct from that of the promonocyte in having a round contour without pseudopods, a nuclear to cytoplasmic ratio greater than one, a cytoplasm that contains many polyribosomes, a few small granules, and a small Golgi complex surrounded by a few short strips of rough endoplasmic reticulum.
最近开发了一种新方法，可在玻璃表面的液体培养基中培养骨髓细胞。在这些培养物中会形成两种集落，即单核吞噬细胞集落和粒细胞集落。本研究的主要目的是对单核吞噬细胞集落中的细胞进行超微结构研究。单核吞噬细胞集落的结构似乎与粒细胞集落有很大不同，因为在粒细胞集落中，细胞紧密聚集在一起，而在单核吞噬细胞集落中，细胞分布较为松散，中心细胞密度最高，周边细胞呈星状排列。然而，这两种集落完全独立生长，未观察到混合集落。电子显微镜显示，单核吞噬细胞集落中有三种类型的细胞，即原单核细胞、幼单核细胞和巨噬细胞。集落中的幼单核细胞和巨噬细胞的超微结构与直接从小鼠分离的相同类型细胞相似。原单核细胞是单核吞噬细胞集落中最不成熟的细胞，此前尚未被描述过。该细胞的超微结构与幼单核细胞明显不同，其轮廓呈圆形，无伪足，核质比大于1，细胞质中含有许多多核糖体、一些小颗粒以及被几条短的粗面内质网包围的小高尔基体复合体。
