Azuma Hiroshi, Sawada Shoichi, Takeuchi Shunji, Higashiyama Kasumi, Kakigi Akinobu, Takeda Taizo
Department of Otolaryngology, Kochi Medical School, Japan.
Neuroreport. 2003 Mar 3;14(3):423-5. doi: 10.1097/00001756-200303030-00025.
Histamine may have physiological functions in the inner ear. Histamine receptors, however, have not yet been identified in the mammalian cochlea. We determined, using reverse transcription polymerase chain reaction (RT-PCR), that H1, H2, and H3 histamine receptor mRNA is expressed in the rat cochlea. To investigate the distribution of each receptor, the cochlea was divided into three parts: the lateral portion, the medial portion, and the modiolus. The mRNA for each receptor subtype was detected in the modiolus but not in the lateral and medial portions of the cochlea. This is the first evidence of H1, H2, and H3 histamine receptor mRNA in the rat cochlea. These findings support the suggestion that histamine may play a physiological role in the cochlea.
组胺可能在内耳具有生理功能。然而，在哺乳动物的耳蜗中尚未鉴定出组胺受体。我们使用逆转录聚合酶链反应（RT-PCR）确定，H1、H2和H3组胺受体mRNA在大鼠耳蜗中表达。为了研究每种受体的分布，将耳蜗分为三个部分：外侧部分、内侧部分和蜗轴。在蜗轴中检测到了每种受体亚型的mRNA，但在耳蜗的外侧和内侧部分未检测到。这是大鼠耳蜗中存在H1、H2和H3组胺受体mRNA的首个证据。这些发现支持了组胺可能在耳蜗中发挥生理作用的观点。
