Shiokawa Satoshi, Matsumato Naoyuki, Nishimura Junji
Division of Clinical Immunology, Department of Immunobiology and Neuroscience, Medical Institute of Bioregulation, Kyushu University, Beppu, Oita, Japan.
Scand J Rheumatol. 2003;32(1):12-8. doi: 10.1080/03009740310000346.
To investigate the clonal characteristics of B cells in the synovial membranes of rheumatoid arthritis (RA) patients.
The clonality of B cells at separate sites of the synovial membrane and other tissues from RA patients were investigated by a reverse transcriptase-polymerase chain reaction with a VH framework 3 consensus primer and a subsequent single-strand conformation polymorphism analysis.
Several dominant bands were observed in all synovial membrane samples and some of the dominants bands were common among the 2 or 3 separate regions of each synovial sample. The persistent existence of clonal B cells was observed in metachronous synovial fluid samples.
Infiltrating B cells are oligoclonal and antigen-driven mechanisms may play a role in the generation of clonal B cells in RA synovium. The stable presence of B cell clones in synovial fluid suggests the involvement of these clones in the perpetuation of the chronic inflammation in RA.
研究类风湿关节炎（RA）患者滑膜中B细胞的克隆特征。
采用逆转录聚合酶链反应，使用VH框架3共有引物，随后进行单链构象多态性分析，研究RA患者滑膜不同部位及其他组织中B细胞的克隆性。
在所有滑膜样本中均观察到几条优势条带，且部分优势条带在每个滑膜样本的2或3个不同区域中是共有的。在不同时间采集的滑液样本中观察到克隆性B细胞持续存在。
浸润的B细胞是寡克隆的，抗原驱动机制可能在RA滑膜中克隆性B细胞的产生中起作用。滑膜液中B细胞克隆的稳定存在表明这些克隆参与了RA慢性炎症的持续存在。
