Takatori T, Phillips F C, Shimasaki H, Privett O S
Lipids. 1976 Apr;11(4):272-80. doi: 10.1007/BF02544053.
Groups of rats were fed from weaning with diets containing 5% by wt of hydrogenated coconut oil (HCO), safflower oil, or a concentrate of ethyl elaidate and linolelaidate (TRANS) as the sole source of dietary fat. Fatty acid composition of the lipid classes from serum, liver, heart, and kidney was determined, and the serum lecithin: cholesterol acyl transferase (LCAT) activities were assayed for each animal. Serum LCAT activity was increased by both the HCO and TRANS diets in the early stages of the development of an essential fatty acid (EFA) deficiency but was suppressed in the animals of the TRANS group as they became older. The HCO and TRANS groups exhibited changes in tissue lipid fatty acid composition, as well as reduced growth, characteristic of an EFA deficiency. Coversion of oleic acid to eicosatrienoic acid was impaired in the animals fed the TRANS diet, greatly increasing the octadecenoic acid content of the tissue lipids at the expense of eicosatrienoic acid. The TRANS diet also suppressed incorporation of eicosatrienoic acid into cholesteryl esters of tissue and serum, indicating that, when fed as the sole source of unsaturated fat, trans fatty acids influenced the metabolism of unsaturated fatty acids and cholesterol.
将大鼠从断奶起分为几组，分别用含5%（重量）氢化椰子油（HCO）、红花油或反油酸乙酯和亚油酸乙酯浓缩物（反式脂肪酸）的日粮喂养，这些油脂作为日粮中唯一的脂肪来源。测定了血清、肝脏、心脏和肾脏中各类脂质的脂肪酸组成，并对每只动物的血清卵磷脂：胆固醇酰基转移酶（LCAT）活性进行了测定。在必需脂肪酸（EFA）缺乏症发展的早期阶段，HCO和反式脂肪酸日粮均使血清LCAT活性升高，但随着年龄增长，反式脂肪酸组动物的该活性受到抑制。HCO组和反式脂肪酸组均表现出组织脂质脂肪酸组成的变化以及生长减缓，这是EFA缺乏的特征。喂食反式脂肪酸日粮的动物中，油酸向二十碳三烯酸的转化受损，组织脂质中的十八碳烯酸含量大幅增加，而二十碳三烯酸含量减少。反式脂肪酸日粮还抑制了二十碳三烯酸掺入组织和血清胆固醇酯中，这表明，当作为不饱和脂肪的唯一来源喂食时，反式脂肪酸会影响不饱和脂肪酸和胆固醇的代谢。
