Moore C E, Alfin-Slater R B, Aftergood L
J Nutr. 1980 Nov;110(11):2284-90. doi: 10.1093/jn/110.11.2284.
An investigation was undertaken to study the effect of trans isomers of octadecenoic (18:1) and octadecadienoic (18:2) acids on serum lecithin:cholesterol acyltransferase (LCAT). Male rats of the Wistar strain were fed diets containing 15% fat for 9 months. The fat diets were either hydrogenated fat mixtures containing trans fatty acids, corn oil or lard. At the end of 9 months, rats were killed and serum cholesterol levels and LCAT activity were measured. It was found that free and total serum cholesterol levels were decreased when animals were fed trans fatty acids when compared with animals fed corn oil or lard. Although in vitro studies indicated that the percentage of cholesterol esterified in serum was not affected by the presence of dietary trans fatty acids, LCAT activity generally decreased when trans fatty acids were fed to rats for 9 months. In this study a negative correlation was found between serum free cholesterol and LCAT activity. Depressed total LCAT activity (mumoles of cholesterol esterified per hour per liter of serum) may have been related to a reduced amount of substrate (free cholesterol).
开展了一项研究，以探讨十八碳烯酸（18:1）和十八碳二烯酸（18:2）的反式异构体对血清卵磷脂胆固醇酰基转移酶（LCAT）的影响。将Wistar品系的雄性大鼠喂食含15%脂肪的日粮9个月。脂肪日粮分别为含反式脂肪酸的氢化脂肪混合物、玉米油或猪油。9个月末，处死大鼠并测量血清胆固醇水平和LCAT活性。结果发现，与喂食玉米油或猪油的动物相比，喂食反式脂肪酸的动物血清游离胆固醇和总胆固醇水平降低。尽管体外研究表明，日粮中反式脂肪酸的存在不影响血清中胆固醇酯化的百分比，但给大鼠喂食反式脂肪酸9个月后，LCAT活性通常会降低。在本研究中，发现血清游离胆固醇与LCAT活性之间呈负相关。总LCAT活性降低（每升血清每小时胆固醇酯化的微摩尔数）可能与底物（游离胆固醇）量减少有关。
