van Stipdonk Marianne J B, Hardenberg Gijs, Bijker Martijn S, Lemmens Edward E, Droin Nathalie M, Green Douglas R, Schoenberger Stephen P
Division of Cellular Immunology, La Jolla Institute for Allergy and Immunology, San Diego, CA 92024, USA.
Nat Immunol. 2003 Apr;4(4):361-5. doi: 10.1038/ni912. Epub 2003 Mar 17.
The initial encounter with an antigen-presenting cell (APC) is the primary force behind the expansion, differentiation and survival of naive T cells. Using an APC that permits temporal control of priming, we examined whether the duration of antigenic stimulation can influence the functional development of CD8+ cytotoxic T lymphocytes (CTLs) in vivo. Whereas CTLs given a 4-h stimulus underwent an abortive clonal expansion with transient surface CD25 expression, those given a 20-h stimulus sustained CD25 up-regulation, proliferated extensively, and efficiently mediated destruction of peripheral target tissues. Our results show that an instructional program preceding the first cell division integrates differences in signal strength into the decision to activate versus tolerize specific CTL clones.
与抗原呈递细胞（APC）的初次接触是幼稚T细胞扩增、分化和存活背后的主要驱动力。我们使用一种允许对启动进行时间控制的APC，研究了抗原刺激的持续时间是否会影响体内CD8 + 细胞毒性T淋巴细胞（CTL）的功能发育。给予4小时刺激的CTL经历了短暂的克隆扩增，表面CD25表达短暂，而给予20小时刺激的CTL持续上调CD25，广泛增殖，并有效介导外周靶组织的破坏。我们的结果表明，第一次细胞分裂之前的指导程序将信号强度的差异整合到激活与耐受特定CTL克隆的决策中。
