Sesti Federico, Rajan Sindhu, Gonzalez-Colaso Rosana, Nikolaeva Natalia, Goldstein Steve A N
Department of Pediatrics, Yale University School of Medicine, Boyer Center for Molecular Medicine, 295 Congress Avenue, New Haven, Connecticut 06536, USA.
Nat Neurosci. 2003 Apr;6(4):353-61. doi: 10.1038/nn1028.
MVP, a Methanococcus jannaschii voltage-gated potassium channel, was cloned and shown to operate in eukaryotic and prokaryotic cells. Like pacemaker channels, MVP opens on hyperpolarization using S4 voltage sensors like those in classical channels activated by depolarization. The MVP S4 span resembles classical sensors in sequence, charge, topology and movement, traveling inward on hyperpolarization and outward on depolarization (via canaliculi in the protein that bring the extracellular and internal solutions into proximity across a short barrier). Thus, MVP opens with sensors inward indicating a reversal of S4 position and pore state compared to classical channels. Homologous channels in mammals and plants are expected to function similarly.
MVP是一种詹氏甲烷球菌电压门控钾通道，已被克隆并证明可在真核细胞和原核细胞中发挥作用。与起搏器通道一样，MVP利用S4电压传感器在超极化时开放，这些传感器类似于那些由去极化激活的经典通道中的传感器。MVP的S4跨膜区在序列、电荷、拓扑结构和运动方面类似于经典传感器，在超极化时向内移动，在去极化时向外移动（通过蛋白质中的小管，使细胞外和细胞内溶液在短屏障处靠近）。因此，MVP在传感器向内时开放，这表明与经典通道相比，S4位置和孔状态发生了反转。预计哺乳动物和植物中的同源通道也会有类似的功能。
