Machii Ryoko, Matsuda Kazuyuki, Hiratsuka Nobuo, Sugimoto Kayo, Hotta Osamu, Itoh Yoshihisa, Yoshida Hiroshi, Shiba Kiyoko
Analytical Chemistry Laboratory, Graduate School of Allied Health Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
J Clin Lab Anal. 2003;17(2):37-43. doi: 10.1002/jcla.10065.
Cellulose acetate membrane electrophoresis with colloidal silver stain re-vealed that the width of the albumin fraction in IgA nephropathy (IgAN) urine before treatment was significantly expanded. This phenomenon was not shown in IgAN urine after treatment or in non-IgAN urine. There was a reverse correlation between the width of the albumin fraction and the albumin con-centration in IgAN urine. By immuno-fixation, Tamm-Horsfall protein (THP) was located in the same position as the albumin band in IgAN urine before treatment; however, in the urine of a healthy subject it was located in the same position as alpha(1)-globulin. By ELISA, the THP-albumin complex concentration in IgAN urine before treatment was significantly higher than in the other two diseases. The width of the albumin fraction and the sodium ion concentra-tion of the urine were significantly correlated. The THP/albumin ratio in IgAN urine before treatment was significantly higher than in the other two groups. This suggests that the characteristic expanded width of albumin found by immunofixation indicates a THP-albumin complex, and that the sodium concentration of urine is involved in the formation of this complex.
醋酸纤维素膜电泳结合胶体银染色显示，治疗前IgA肾病（IgAN）患者尿液中白蛋白组分的宽度显著增宽。这种现象在治疗后的IgAN患者尿液或非IgAN患者尿液中未出现。IgAN患者尿液中白蛋白组分的宽度与白蛋白浓度呈负相关。通过免疫固定法发现，治疗前IgAN患者尿液中Tamm-Horsfall蛋白（THP）与白蛋白条带位于同一位置；然而，在健康受试者尿液中，THP与α1球蛋白位于同一位置。通过酶联免疫吸附测定（ELISA），治疗前IgAN患者尿液中THP-白蛋白复合物浓度显著高于其他两种疾病。尿液中白蛋白组分的宽度与钠离子浓度显著相关。治疗前IgAN患者尿液中THP/白蛋白比值显著高于其他两组。这表明免疫固定法发现的白蛋白特征性增宽提示存在THP-白蛋白复合物，且尿液中的钠浓度参与了该复合物的形成。
