Fiers W, Contreras R, Duerinck F, Haegeman G, Iserentant D, Merregaert J, Min Jou W, Molemans F, Raeymaekers A, Van den Berghe A, Volckaert G, Ysebaert M
Nature. 1976 Apr 8;260(5551):500-7. doi: 10.1038/260500a0.
Bacteriophage MS2 RNA is 3,569 nucleotides long. The nucleotide sequence has been established for the third and last gene, which codes for the replicase protein. A secondary structure model has also been proposed. Biological properties, such as ribosome binding and codon interactions can now be discussed on a molecular basis. As the sequences for the other regions of this RNA have been published already, the complete, primary chemical structure of a viral genome has now been established.
噬菌体MS2 RNA长3569个核苷酸。已确定了编码复制酶蛋白的第三个也是最后一个基因的核苷酸序列。还提出了一种二级结构模型。现在可以在分子基础上讨论诸如核糖体结合和密码子相互作用等生物学特性。由于该RNA其他区域的序列已经发表,因此现已确定了病毒基因组完整的一级化学结构。