Zilber Moshe, Friedman Ziva, Shapiro Hava, Shapira Shirley, Radnay Judith, Ellis Martin H
Department of Medicine D, Meir Hospital, Kfar Saba, Israel.
Clin Appl Thromb Hemost. 2003 Jan;9(1):39-44. doi: 10.1177/107602960300900105.
Plasma-reduced platelet concentrates are commonly administered to prevent febrile transfusion reactions and to avoid fluid overload in neonates. Because little is known about the influence of centrifugation and resuspension on functional aspects of platelets, we examined the effects of plasma-reduction on platelet aggregation and platelet-dependent thrombin generation. Our results show that plasma reduction and resuspension of the platelet pellet in saline or plasma results in a significant reduction in platelet aggregation to a combination of the platelet agonists adenosine diphosphate and epinephrine (p < 0.001). In contrast, when a combination of the more potent agonists collagen and thrombin was used, platelet aggregation was maintained. Likewise, no decline was observed in platelet-dependent thrombin generation as measured by the functional prothrombinase assay or Annexin V binding. We conclude that centrifugation and resuspension of platelets to render the concentrate plasma-free, as a routine procedure in blood banking, variably affects in vitro platelet aggregability but does not significantly affect platelet-dependent thrombin generation.
血浆减少的血小板浓缩物通常用于预防新生儿发热性输血反应并避免液体超负荷。由于对于离心和重悬对血小板功能方面的影响了解甚少，我们研究了血浆减少对血小板聚集和血小板依赖性凝血酶生成的影响。我们的结果表明，血小板沉淀在生理盐水或血浆中的血浆减少和重悬导致血小板对血小板激动剂二磷酸腺苷和肾上腺素组合的聚集显著减少（p < 0.001）。相比之下，当使用更强效的激动剂胶原蛋白和凝血酶组合时，血小板聚集得以维持。同样，通过功能性凝血酶原酶测定或膜联蛋白V结合测量，未观察到血小板依赖性凝血酶生成下降。我们得出结论，作为血库中的常规程序，对血小板进行离心和重悬以使浓缩物无血浆，会不同程度地影响体外血小板聚集能力，但不会显著影响血小板依赖性凝血酶生成。
