Shapira S, Friedman Z, Shapiro H, Presseizen K, Radnay J, Ellis M H
Department of Medicine "B", Meir Hospital, Kfar Saba, Israel.
Transfusion. 2000 Oct;40(10):1257-63. doi: 10.1046/j.1537-2995.2000.40101257.x.
Platelet concentrates (PCs) derived from whole blood and stored under standard blood bank conditions undergo changes that are referred to as the platelet storage lesion. This study assesses the effect of PC preparation and storage on the distribution of phosphatidylserine (PS) in the platelet membrane and the effect that this distribution may have on the thrombogenic potential of stored PCs.
Fresh platelets and PCs donated by healthy donors were obtained. PCs derived from platelet-rich plasma were studied on Day 1, Day 3, and Day 6 of storage under blood bank conditions.
Platelet aggregation after exposure to the platelet agonists ADP and epinephrine singly declined progressively, but, when ADP and epinephrine in combination and collagen and thrombin in combination were used as agonists, the decline in platelet aggregation was less marked. PS expression as measured by Annexin V binding (mean and SD) was 2.02 +/- 0.93 percent in fresh platelet samples and increased to 5.39 +/- 4.2 percent on Day 1, 22. 1 +/- 7.1 percent on Day 3, and 39.5 +/- 12.1 percent on Day 6. Platelet prothrombinase activity (mean +/- SD) as measured by thrombin generation increased from 1.49 +/- 0.7 micro per mL in fresh platelet samples to 3.68 +/- 1.1 micro per mL in Day 1 platelets (p<0.001), 5.15 +/- 2.5 micro per mL in Day 3 platelets (p<0.001), and 4.65 +/- 2.48 micro per mL in Day 6 platelets (p<0. 001).
These results show that PS expression increases after preparation of PCs from platelet-rich plasma and rises progressively during platelet storage under blood bank conditions. Furthermore, the greater PS expression is associated with increased platelet- dependent thrombin-generating capacity.
从全血中提取并在标准血库条件下储存的血小板浓缩物（PCs）会发生变化，即所谓的血小板储存损伤。本研究评估了PC制备和储存对血小板膜中磷脂酰丝氨酸（PS）分布的影响，以及这种分布可能对储存的PCs血栓形成潜力产生的影响。
获取健康供体捐献的新鲜血小板和PCs。对富含血小板血浆来源的PCs在血库条件下储存的第1天、第3天和第6天进行研究。
单独暴露于血小板激动剂ADP和肾上腺素后，血小板聚集逐渐下降，但是，当联合使用ADP和肾上腺素以及联合使用胶原蛋白和凝血酶作为激动剂时，血小板聚集的下降不太明显。通过膜联蛋白V结合测量的PS表达（平均值和标准差）在新鲜血小板样本中为2.02±0.93%，在第1天增加到5.39±4.2%，第3天为22.1±7.1%，第6天为39.5±12.1%。通过凝血酶生成测量的血小板凝血酶原酶活性（平均值±标准差）从新鲜血小板样本中的1.49±0.7微摩尔/毫升增加到第1天血小板中的3.68±1.1微摩尔/毫升（p<0.001），第3天血小板中的5. ADP和肾上腺素以及联合使用胶原蛋白和凝血酶作为激动剂时，血小板聚集的下降不太明显。通过膜联蛋白V结合测量的PS表达（平均值和标准差）在新鲜血小板样本中为2.02±0.93%，在第1天增加到5.39±4.2%，第3天为22.1±7.1%，第6天为39.5±12.1%。通过凝血酶生成测量的血小板凝血酶原酶活性（平均值±标准差）从新鲜血小板样本中的 = /毫升增加到第1天血小板中的3.68±1.1微摩尔/毫升（p<0.001），第3天血小板中的5.15±2.5微摩尔/毫升（p<0.001），第6天血小板中的4.65±2.48微摩尔/毫升（p<0.001）。
这些结果表明，从富含血小板血浆制备PCs后PS表达增加，并且在血库条件下血小板储存期间逐渐上升。此外，更高的PS表达与血小板依赖性凝血酶生成能力增加相关。 1±2.5微摩尔/毫升（p<0.001），第6天血小板中的4.65±2.48微摩尔/毫升（p<0.001）。
这些结果表明，从富含血小板血浆制备PCs后PS表达增加，并且在血库条件下血小板储存期间逐渐上升。此外，更高的PS表达与血小板依赖性凝血酶生成能力增加相关。
