Murata Yuzo, Masuko Sadahiko
Division of Histology and Neuroanatomy, Department of Anatomy and Physiology, Saga Medical School, Nabeshima 5-1-1, Saga 849-8501, Japan.
Brain Res Dev Brain Res. 2003 Mar 14;141(1-2):91-9. doi: 10.1016/s0165-3806(02)00646-6.
The prenatal and postnatal development of NADPH-diaphorase (NADPH-d)/neuronal nitric oxide synthase (nNOS) positive neurons was studied in the striatum of rats. NADPH-d was demonstrated enzyme histochemically and nNOS immunohistochemically using a polyclonal antibody. NADPH-d neurons appeared in the ventrolateral part of the striatum on embryonic day 18 (E18). Thereafter, the number of NADPH-d neurons increased and began to distribute homogeneously in the striatum. The density of NADPH-d neurons became highest at postnatal day 5 (P5) and then decreased as the volume of the striatum continued to increase. The number of NADPH-d neurons reached its peak around 3-4 weeks after birth. The sizes of NADPH-d neurons were measured. The NADPH-d neurons grew larger until P14 (mean area 260 microm(2)) and became smaller thereafter (mean area 170 microm(2)). Patches of high NADPH-d activity and tyrosine hydroxylase (TH) immunoreactivity were also examined in the developing striatum. The distributions of NADPH-d patches overlapped with those of TH-immunoreactive patches by P10. The spatiotemporal appearance of nNOS and overlapping of nNOS patchy distribution with TH point to an important role of NO and to an interaction between nNOS and DA fibers during development of the striatum.
研究了大鼠纹状体中烟酰胺腺嘌呤二核苷酸磷酸二酯酶（NADPH-d）/神经元型一氧化氮合酶（nNOS）阳性神经元的产前和产后发育情况。采用酶组织化学方法显示NADPH-d，并使用多克隆抗体通过免疫组织化学方法显示nNOS。NADPH-d神经元在胚胎第18天（E18）出现在纹状体的腹外侧部分。此后，NADPH-d神经元数量增加，并开始在纹状体中均匀分布。NADPH-d神经元密度在出生后第5天（P5）达到最高，然后随着纹状体体积持续增加而降低。NADPH-d神经元数量在出生后3 - 4周左右达到峰值。对NADPH-d神经元的大小进行了测量。NADPH-d神经元在P14之前逐渐变大（平均面积260平方微米），之后变小（平均面积170平方微米）。还在发育中的纹状体中检查了高NADPH-d活性和酪氨酸羟化酶（TH）免疫反应性的斑块。到P10时，NADPH-d斑块的分布与TH免疫反应性斑块的分布重叠。nNOS的时空出现以及nNOS斑块状分布与TH的重叠表明一氧化氮在纹状体发育过程中起重要作用，并且nNOS与多巴胺纤维之间存在相互作用。
