Tay David, Diao Yun-Cheng, Xiao Yue-Mei, So Kwok-Fai
Department of Anatomy, Faculty of Medicine, University of Hong Kong, Hong Kong, China.
J Comp Neurol. 2002 May 13;446(4):342-8. doi: 10.1002/cne.10201.
The histochemical method was used to investigate the postnatal development of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) -positive neurons in retinas of the golden hamster. NADPH-d-positive neurons were discernible in the retina at postnatal day (P)1. From P4 onward to adulthood, when the retina acquired its laminated characteristics, NADPH-d- positive neurons were observed in the inner nuclear layer (INL) and the ganglion cell layer (GCL). Results showed that NADPH-d-positive neurons in INL and GCL followed different time courses and patterns in their development. NADPH-d-positive neurons in INL underwent a sharp increase from P4 to P8 (3.6-fold), followed by a decrease to 46% of the maximum at P12. This value was maintained relatively constant to the adult level. The mean diameters of NADPH-d-positive neurons in INL, which were smaller than those in the GCL for all ages, increased from P8 to P12 and from P20 to adulthood. As for neurons in the GCL, the increase in cell number was not so apparent for the earlier postnatal days until P20; thereafter, an obvious increase to the adult level was observed. The mean diameters of the NADPH-d-positive cell bodies in the GCL increased with age, except for P16-P20, during which time there was a slight and insignificant decrease. The tendency of changes in cell density was basically similar to that of the total number for both the INL and the GCL. Between P12 and P20, the density distribution map of the NADPH-d-positive neurons underwent dramatic changes: The highest density shifted from the upper central retina at the earlier postnatal days to the lower central retina in the adult. The two waves of increase in NADPH-d-positive neurons coincide with the process of axonal elongation and synaptogenesis and the acquisition of visual function and experience. It is suggested that these NADPH-d-positive neurons are related to these two developmental events.
采用组织化学方法研究金黄仓鼠视网膜中烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）阳性神经元的出生后发育情况。出生后第1天（P1），视网膜中可辨别出NADPH-d阳性神经元。从P4至成年期，当视网膜获得其分层特征时，在内核层（INL）和神经节细胞层（GCL）中观察到NADPH-d阳性神经元。结果表明，INL和GCL中的NADPH-d阳性神经元在发育过程中遵循不同的时间进程和模式。INL中的NADPH-d阳性神经元从P4到P8急剧增加（3.6倍），随后在P12时降至最大值的46%。该值相对稳定地维持到成年水平。INL中NADPH-d阳性神经元的平均直径在各年龄段均小于GCL中的，从P8到P12以及从P20到成年期均有所增加。至于GCL中的神经元，出生后早期直至P20，细胞数量的增加并不明显；此后，观察到明显增加至成年水平。GCL中NADPH-d阳性细胞体的平均直径随年龄增加，除了P16 - P20期间有轻微且不显著的下降。细胞密度的变化趋势与INL和GCL的总数基本相似。在P12和P20之间，NADPH-d阳性神经元的密度分布图发生了显著变化：出生后早期最高密度从视网膜中央上部转移到成年期的视网膜中央下部。NADPH-d阳性神经元的两波增加与轴突伸长和突触形成过程以及视觉功能和经验的获得相吻合。提示这些NADPH-d阳性神经元与这两个发育事件有关。
