Ozaki Toshifumi, Neumann Thomas, Wai Daniel, Schäfer Karl-Ludwig, van Valen Franz, Lindner Norbert, Scheel Christina, Böcker Wermer, Winkelmann Winfried, Dockhorn-Dworniczak Barbara, Horst Jürgen, Poremba Christopher
Department of Orthopaedics, Westfälische Wilhelms-University, Münster, Germany.
Cancer Genet Cytogenet. 2003 Jan 15;140(2):145-52. doi: 10.1016/s0165-4608(02)00685-4.
We characterized the chromosomal alterations in eight osteosarcoma cell lines (OST, HOS, U-2 OS, ZK-58, MG-63, SJSA-1, Saos-2, and MNNG) by comparative genomic hybridization (CGH); gains and losses of DNA sequences were defined as chromosomal regions with a fluorescence ratio, wherein all of the 95% confidence interval was above 1.25 and below 0.75, respectively. In four of 8 cell lines, multicolor karyotyping (MK) was added. CGH revealed the average number of aberrations per cell line was 20.8 (range: 10-31); the average numbers of gains and losses were 11.1 and 9.6, respectively. The frequent gains were identified on 1p21 approximately q24, 1q25-q31, 7p21, 7q31, 8q23 approximately q24, and 14q21; frequent losses were at 18q21 approximately q22, 18q12, 19p, and 3p12 approximately p14. High-level gains were observed on 8q23 approximately q24, 5p, and 1p21 approximately p22. MK revealed the most common translocations in the four cell lines were t(8;9), t(1;3), t(3;5), t(1;13), t(2;6), t(3; 17), t(1;15), t(10;20), and t(6;20). Chromosomes 1, 3, 8, 9, and 20 were most frequently involved in translocation events. The concordance rate of aberrations in CGH and translocations in MK was 76%. MK was useful to identify the chromosomal alterations and as a supplement to the CGH results in three of four chromosomes.
我们通过比较基因组杂交（CGH）对8种骨肉瘤细胞系（OST、HOS、U - 2 OS、ZK - 58、MG - 63、SJSA - 1、Saos - 2和MNNG）的染色体改变进行了特征分析；DNA序列的增加和减少被定义为荧光比率对应的染色体区域，其中所有95%置信区间分别高于1.25和低于0.75。在8个细胞系中的4个中，增加了多色核型分析（MK）。CGH显示每个细胞系的平均畸变数量为20.8（范围：10 - 31）；增加和减少的平均数量分别为11.1和9.6。常见的增加区域位于1p21大约到q24、1q25 - q31、7p21、7q31、8q23大约到q24和14q21；常见的减少区域位于18q21大约到q22、18q12、19p和3p12大约到p14。在8q23大约到q24、5p和1p21大约到p22观察到高水平的增加。MK显示4个细胞系中最常见的易位为t(8;9)、t(1;3)、t(3;5)、t(1;13)、t(2;6)、t(3;17)、t(1;15)、t(10;20)和t(6;20)。染色体1、3、8、9和20最常参与易位事件。CGH中的畸变与MK中的易位的一致率为76%。MK有助于识别染色体改变，并作为CGH结果在四条染色体中的三条上的补充。
