Backovic Marija, Gettins Peter G W
Department of Biochemistry and Molecular Biology, College of Medicine, University of Illinois at Chicago, Chicago, Illinois 60612-4316, USA.
J Proteome Res. 2002 Jul-Aug;1(4):367-73. doi: 10.1021/pr025515z.
Complete sequences were determined for frog, turtle, and ostrich antithrombins. Protein sequence comparisons with the other 10 known antithrombin sequences and with sequences of other serpins have provided striking evidence for the conservation of the heparin activation mechanism and new insight into those residues important for heparin binding, for heparin activation, and for reactive center loop function, as well as an indication of which glycosylation sites might be needed for function. Importantly, an understanding of, as yet, poorly understood antithrombin-protein interactions will be greatly aided by this expanded database and comparative analysis.
已测定青蛙、乌龟和鸵鸟抗凝血酶的完整序列。将这些蛋白质序列与其他10种已知抗凝血酶序列以及其他丝氨酸蛋白酶抑制剂的序列进行比较，为肝素激活机制的保守性提供了显著证据，并对肝素结合、肝素激活、反应中心环功能重要的残基有了新的认识，还指出了哪些糖基化位点可能对功能是必需的。重要的是，这个扩展的数据库和比较分析将极大地有助于理解目前仍了解甚少的抗凝血酶与蛋白质的相互作用。
