Ciuraszkiewicz Justyna, Olczak Mariusz, Watorek Wiesław
Laboratory of Biochemistry, Institute of Biochemistry and Molecular Biology, Wroclaw University, Tamka 2, 50-137 Wroclaw, Poland.
Comp Biochem Physiol B Biochem Mol Biol. 2006 Jul;144(3):301-10. doi: 10.1016/j.cbpb.2006.03.003. Epub 2006 Mar 14.
Transferrins form an important class of iron-binding proteins widely distributed in the physiological fluids of vertebrates and invertebrates. In vertebrates they are present mostly in serum as serotransferrins. In birds and reptiles transferrins are also found in eggs as ovotransferrins. However, until now only chicken and duck ovotransferrin sequences have been published. This paper presents data on the purification, biochemical analysis, cloning and sequencing of ovotransferrins from red-eared turtle, African ostrich and turkey, revealing their significant homology with other known ovotransferrin sequences. The proteins were purified by size-exclusion and anion-exchange chromatography. Isoelectric points, iron-saturated and iron-free spectra, as well as the mRNA nucleotide sequences of 2,409 nt (ORF: 2,106 nt encoding a 701-amino-acid polypeptide; ), 2,418 nt (ORF 2,118 nt encoding a 705-amino-acid polypeptide; ), and 2,397 nt (ORF: 2,118 nt encoding a 705-amino-acid polypeptide; ) were determined for ostrich (OtrF), red-eared turtle (TtrF), and turkey (MtrF) ovotransferrin, respectively.
转铁蛋白是一类重要的铁结合蛋白，广泛分布于脊椎动物和无脊椎动物的生理体液中。在脊椎动物中，它们主要以血清转铁蛋白的形式存在于血清中。在鸟类和爬行动物中，转铁蛋白也以卵转铁蛋白的形式存在于卵中。然而，到目前为止，仅公布了鸡和鸭的卵转铁蛋白序列。本文展示了关于红耳龟、非洲鸵鸟和火鸡卵转铁蛋白的纯化、生化分析、克隆和测序的数据，揭示了它们与其他已知卵转铁蛋白序列的显著同源性。这些蛋白质通过尺寸排阻色谱和阴离子交换色谱进行纯化。分别测定了鸵鸟（OtrF）、红耳龟（TtrF）和火鸡（MtrF）卵转铁蛋白的等电点、铁饱和和无铁光谱，以及2409 nt（开放阅读框：2106 nt，编码一个701个氨基酸的多肽）、2418 nt（开放阅读框2118 nt，编码一个705个氨基酸的多肽）和2397 nt（开放阅读框：2118 nt，编码一个705个氨基酸的多肽）的mRNA核苷酸序列。
