Pascolo Lorella, Fernetti Cristina, Pirulli Doroti, Crovella Sergio, Amoroso Antonio, Tiribelli Claudio
Centro Studi Fegato at AREA Science Park and Dipartimento BBCM, Universitá di Trieste, Trieste, Italy.
Biochem Biophys Res Commun. 2003 Mar 28;303(1):259-65. doi: 10.1016/s0006-291x(03)00327-9.
The placenta is a multifunctional organ that protects the fetus from toxic compounds and the MRPs contribute to this function. The expression of MRP1, MRP2, MRP3, and MRP5 was compared in human placental tissue and in BeWo cells by real-time RT-PCR analysis; protein expression was assessed by Western blot. MRP1 and MRP3 were the most abundantly expressed genes in placenta but only MRP1 was highly expressed in the BeWo cells. Expression of MRP1 increased 4-fold in the third as compared with first trimester placental samples, and increased 20-fold with polarization of BeWo cells. MRP2, MRP3, and MRP5 were weakly expressed both in placenta and BeWo cells. Protein expression followed mRNA quantification for MRP1 and MRP5 but not for MRP2 and MRP3. These data indicated that MRP1 and MRP5 increase with trophoblast maturation, suggesting a particular role for these proteins in the organ functional development.
胎盘是一个多功能器官，可保护胎儿免受有毒化合物的侵害，多药耐药相关蛋白（MRPs）有助于这一功能。通过实时逆转录聚合酶链反应（RT-PCR）分析比较了人胎盘组织和BeWo细胞中MRP1、MRP2、MRP3和MRP5的表达；通过蛋白质印迹法评估蛋白质表达。MRP1和MRP3是胎盘中表达最丰富的基因，但只有MRP1在BeWo细胞中高表达。与孕早期胎盘样本相比，MRP1在孕晚期胎盘样本中的表达增加了4倍，并且随着BeWo细胞的极化增加了20倍。MRP2、MRP3和MRP5在胎盘和BeWo细胞中均弱表达。MRP1和MRP5的蛋白质表达与mRNA定量结果一致，但MRP2和MRP3并非如此。这些数据表明，MRP1和MRP5随着滋养层成熟而增加，提示这些蛋白质在器官功能发育中具有特殊作用。
