Nichols K M, Young W P, Danzmann R G, Robison B D, Rexroad C, Noakes M, Phillips R B, Bentzen P, Spies I, Knudsen K, Allendorf F W, Cunningham B M, Brunelli J, Zhang H, Ristow S, Drew R, Brown K H, Wheeler P A, Thorgaard G H
School of Biological Sciences, Washington State University, Pullman, WA, USA.
Anim Genet. 2003 Apr;34(2):102-15. doi: 10.1046/j.1365-2052.2003.00957.x.
Androgenetic doubled haploid progeny produced from a cross between the Oregon State University and Arlee clonal rainbow trout (Oncorhynchus mykiss) lines, used for a previous published rainbow trout map, were used to update the map with the addition of more amplified fragment length polymorphic (AFLP) markers, microsatellites, type I and allozyme markers. We have added more than 900 markers, bringing the total number to 1359 genetic markers and the sex phenotype including 799 EcoRI AFLPs, 174 PstI AFLPs, 226 microsatellites, 72 VNTR, 38 SINE markers, 29 known genes, 12 minisatellites, five RAPDs, and four allozymes. Thirty major linkage groups were identified. Synteny of linkage groups in our map with the outcrossed microsatellite map has been established for all except one linkage group in this doubled haploid cross. Putative homeologous relationships among linkage groups, resulting from the autotetraploid nature of the salmonid genome, have been revealed based on the placement of duplicated microsatellites and type I loci.
由俄勒冈州立大学和阿利克隆虹鳟（Oncorhynchus mykiss）品系杂交产生的雄核发育加倍单倍体后代，曾用于之前发表的虹鳟图谱，此次被用于更新图谱，增加了更多的扩增片段长度多态性（AFLP）标记、微卫星、I型标记和同工酶标记。我们增加了900多个标记，使遗传标记总数达到1359个，包括799个EcoRI AFLP、174个PstI AFLP、226个微卫星、72个VNTR、38个SINE标记、29个已知基因、12个小卫星、5个RAPD和4个同工酶，以及性别表型。共鉴定出30个主要连锁群。在这个加倍单倍体杂交中，除了一个连锁群外，我们图谱中的连锁群与杂交微卫星图谱的同线性关系已经建立。基于重复微卫星和I型基因座的定位，揭示了由于鲑科基因组的同源四倍体性质导致的连锁群之间假定的同源关系。
