Moon Hye-Sung, Park Won I, Sung Sun Hee, Choi Eun-Ah, Chung Hye-Won, Woo Bock Hi
Department of Obstetrics & Gynecology, Ewha Womans University and Medical Research Center, Seoul, Korea.
Gynecol Oncol. 2003 Mar;88(3):289-97. doi: 10.1016/s0090-8258(02)00070-7.
The aim of this study was to determine midkine (MK) and pleiotrophin (PTN) expression in cervical cancer.
Prospective study in tertiary teaching hospital. Normal and cancerous cervical tissues were obtained from healthy women (n = 19) and from patients with cervical cancer (n = 42). The expressions of MK and PTN mRNA and protein were examined by quantitative competitive PCR and by immunohistochemistry. MK and PTN mRNA and protein expressions were examined with respect to tumor stage and size.
The expressions of midkine and pleiotrophin mRNA in cervical cancer were higher than those in the normal cervix (MK, 175.59 +/- 63.3 vs 1.00 +/- 0.18 fmol, respectively; PTN, 3.18 +/- 1.25 vs. 0.86 +/- 0.12 fmol, respectively, P < 0.05), and their expressions were not correlated with cervical cancer stage or size of the tumor. The expressions of MK and PTN protein in cancerous tissue were higher than those in the normal cervix (P < 0.05). Moreover, the protein expression of MK, but not of PTN, correlated with tumor stage and size. The expressions of MK and PTN were not correlated with vascular density.
Our results suggest that increased midkine mRNA and protein expressions are associated with the carcinogenesis of cervical cancer.
本研究旨在确定宫颈癌中中期因子（MK）和多效生长因子（PTN）的表达情况。
在三级教学医院进行前瞻性研究。从健康女性（n = 19）和宫颈癌患者（n = 42）获取正常和癌性宫颈组织。通过定量竞争PCR和免疫组织化学检测MK和PTN mRNA及蛋白的表达。根据肿瘤分期和大小检测MK和PTN mRNA及蛋白的表达。
宫颈癌中中期因子和多效生长因子mRNA的表达高于正常宫颈（MK分别为175.59±63.3与1.00±0.18 fmol；PTN分别为3.18±1.25与0.86±0.12 fmol，P < 0.05），且它们的表达与宫颈癌分期或肿瘤大小无关。癌组织中MK和PTN蛋白的表达高于正常宫颈（P < 0.05）。此外，MK的蛋白表达与肿瘤分期和大小相关，而PTN的蛋白表达与肿瘤分期和大小无关。MK和PTN的表达与血管密度无关。
我们的结果表明，中期因子mRNA和蛋白表达增加与宫颈癌的发生有关。
