Lin Hao, Zhou Qingwen, Wu Weichu, Ma Yulin
Department of Urology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, People's Republic of China.
Department of Urology, Hongsen Hospital of Harbin Medical University, Sanya, Hainan 572000, People's Republic of China.
Onco Targets Ther. 2019 Dec 31;12:11765-11775. doi: 10.2147/OTT.S235134. eCollection 2019.
To determine the role of Midkine (MDK) in non-invasive detection of bladder cancer (Bca) and the relationship with Ki67.
Sixty-five Bca patients and 55 non-Bca patients or healthy volunteers were enrolled and voided urine samples were prospectively obtained on the first day of enrollment. Tissue samples were collected by surgery. MDK and Ki67 expressions were analyzed by immunohistochemistry and Western Blot (WB). Specificity and sensitivity of MDK mRNA testing in the detection of Bca were determined by Receiver Operating Characteristic curve (ROC). The relationship between MDK and Ki67 was also assessed.
MDK was overexpressed in Bca tissues than that in the non-cancer tissues. The specificity and sensitivity for MDK mRNA testing in urine in the identification of Bca was 80% and 72.3%. MDK detected 85.7% of high-grade tumors, 87.5% of muscle-invasive tumors and 79.4% of tumors larger than 3 cm in patients without gross hematuria. Microscopic hematuria may even increase the detection rate of Bca by MDK testing. Furthermore, the correlation of MDK and Ki67 was found positive.
MDK was overexpressed in Bca tissues and positively correlated with Ki67. MDK might be a potential biomarker for the detection of Bca, especially for those without gross hematuria but with microscopic hematuria.
确定中期因子（MDK）在膀胱癌（Bca）非侵入性检测中的作用及其与Ki67的关系。
招募65例Bca患者和55例非Bca患者或健康志愿者，在入组第一天前瞻性采集晨尿样本。手术采集组织样本。采用免疫组织化学和蛋白质免疫印迹法（WB）分析MDK和Ki67的表达。通过受试者工作特征曲线（ROC）确定MDK mRNA检测在Bca检测中的特异性和敏感性。同时评估MDK与Ki67之间的关系。
与非癌组织相比，MDK在Bca组织中过表达。尿液中MDK mRNA检测识别Bca的特异性和敏感性分别为80%和72.3%。在无肉眼血尿的患者中，MDK检测出85.7%的高级别肿瘤、87.5%的肌层浸润性肿瘤和79.4%直径大于3 cm的肿瘤。镜下血尿甚至可能提高MDK检测对Bca的检出率。此外，发现MDK与Ki67呈正相关。
MDK在Bca组织中过表达且与Ki67呈正相关。MDK可能是Bca检测的潜在生物标志物，尤其对于那些无肉眼血尿但有镜下血尿的患者。
