Morgan A W, Keyte V H, Babbage S J, Robinson J I, Ponchel F, Barrett J H, Bhakta B B, Bingham S J, Buch M H, Conaghan P G, Gough A, Green M, Lawson C A, Pease C T, Markham A F, Ollier W E R, Emery P, Worthington J, Isaacs J D
Rheumatology and Rehabilitation Research Unit, University of Leeds, UK.
Rheumatology (Oxford). 2003 Apr;42(4):528-33. doi: 10.1093/rheumatology/keg169.
To develop a robust assay for genotyping the FcgammaRIIIA-158V/F polymorphism and to confirm the putative association between the FcgammaRIIIA-158V allele and rheumatoid arthritis (RA).
This allelic association study examined the FcgammaRIIIA-158V/F polymorphism for association with RA. A novel single-stranded conformational polymorphism assay was used to genotype 828 RA patients and 581 controls from the UK.
The FcgammaRIIIA-158V allele was associated with both RA (P=0.02) and nodules (P=0.04). Individuals homozygous for this higher affinity allele had a significantly increased risk of RA (OR 1.53, 95% CI 1.08-2.18) and the development of nodules (OR 2.20, 95% CI 1.20-4.01). There was no evidence of an interaction with the shared epitope.
We have developed a novel assay to genotype the FcgammaRIIIA-158F/V polymorphism and confirmed that homozygosity for the FcgammaRIIIA-158V allele is associated with UK Caucasian RA, particularly in those individuals with nodules, suggesting FcgammaRIIIA may play a role in determining disease severity or in the development of nodules per se.
开发一种可靠的检测方法用于对FcγRIIIA - 158V/F多态性进行基因分型，并证实FcγRIIIA - 158V等位基因与类风湿关节炎（RA）之间的假定关联。
这项等位基因关联研究检测了FcγRIIIA - 158V/F多态性与RA的关联。使用一种新型单链构象多态性检测方法对来自英国的828例RA患者和581例对照进行基因分型。
FcγRIIIA - 158V等位基因与RA（P = 0.02）和结节（P = 0.04）均相关。携带这种高亲和力等位基因纯合子的个体患RA的风险显著增加（比值比1.53，95%可信区间1.08 - 2.18）以及出现结节的风险增加（比值比2.20，95%可信区间1.20 - 4.01）。没有证据表明与共享表位存在相互作用。
我们开发了一种新型检测方法用于对FcγRIIIA - 158F/V多态性进行基因分型，并证实FcγRIIIA - 158V等位基因纯合子与英国白种人RA相关，特别是在有结节的个体中，提示FcγRIIIA可能在决定疾病严重程度或结节本身的形成中起作用。
