Prada M D, Richards G, Pletscher A
Proc Soc Exp Biol Med. 1976 May;152(1):135-8. doi: 10.3181/00379727-152-39345.
A histo-immunofluorescence technique using anti-actin antibodies has been applied to various subcellular fractions of bovine adrenal medulla and rabbit blood platelets. In the adrenal medulla only the membranes of the chromaffin granules, but not the fractions containing other subcellular particles (microsomes, mitochondria) showed marked immunofluorescence was present in the membranes of the 5-hydroxy-tryptamine organelles as well as in other subcellular particles. It is concluded that in the adrenal medulla, actin is specifically associated with the membranes of the amine storage organelles, whereas in platelets the protein shows a rather general subcellular distribution.
一种使用抗肌动蛋白抗体的组织免疫荧光技术已应用于牛肾上腺髓质和兔血小板的各种亚细胞组分。在肾上腺髓质中，只有嗜铬颗粒的膜呈现明显的免疫荧光，而含有其他亚细胞颗粒（微粒体、线粒体）的组分则没有。5-羟色胺细胞器的膜以及其他亚细胞颗粒中也存在免疫荧光。得出的结论是，在肾上腺髓质中，肌动蛋白与胺储存细胞器的膜特异性相关，而在血小板中，该蛋白质呈现出较为普遍的亚细胞分布。
