Schessner M, Schnorr B
Institut für Veterinär-Anatomie, -Histologie und -Embryologie, Universität Giessen, Federal Republic of Germany.
Cell Tissue Res. 1990 Apr;260(1):109-16. doi: 10.1007/BF00297495.
The distribution of actin filaments in pyloric gland cells of cattle was studied with respect to their functional significance in the process of exocrine secretion by use of rhodamine-phalloidin labelling and immunogold-electron microscopy based on the biotin-streptavidin bridge technique. Actin concentrates on the filamentous network of the luminal-cell cortex. Membranes of secretory vesicles accumulating in the cell cortex are also labelled for actin. The present results support the concept of a barrier function of cortical microfilaments entrapping vesicles and linking them to the cytoskeleton. In addition, intracellular localization of calcium-ATPase activity was determined. Enzyme activity associated with the microfilamentous cortical matrix is supposed to be of cytoskeletal nature indicating participation of myosin (-like) structures in the dynamic secretion event. Deposition on the interior aspect of secretory vesicle membranes points to an ATPase transporting calcium into these organelles and enabling them to participate via storage of the cation in intracellular calcium homeostasis, thereby influencing the functional architecture of the cortical cytoskeleton.
利用罗丹明 - 鬼笔环肽标记法以及基于生物素 - 链霉亲和素桥技术的免疫金电子显微镜技术，研究了牛幽门腺细胞中肌动蛋白丝的分布情况，探讨其在外分泌分泌过程中的功能意义。肌动蛋白集中在管腔细胞皮质的丝状网络上。聚集在细胞皮质中的分泌小泡膜也被标记上肌动蛋白。目前的结果支持皮质微丝具有屏障功能的概念，即捕获小泡并将它们与细胞骨架相连。此外，还测定了钙 - ATP酶活性的细胞内定位。与微丝皮质基质相关的酶活性被认为具有细胞骨架性质，表明肌球蛋白（类）结构参与了动态分泌过程。分泌小泡膜内侧的沉积表明存在一种ATP酶，可将钙转运到这些细胞器中，并通过在细胞内钙稳态中储存阳离子使它们参与其中，从而影响皮质细胞骨架的功能结构。
