Feinstein G, Janoff A
Proc Soc Exp Biol Med. 1976 May;152(1):36-41. doi: 10.3181/00379727-152-39322.
Granules of human peripheral blood leucocytes contain four well-characterized elastase isozymes and one or two slow-moving elastaselike esterases (SE) which have not been as well characterized. SE are capable of hydrolyzing typical elastase synthetic sybstrates such as N-acetyl-dl-alanine-alpha-naphthyl ester (Ac-DL-Ala-1-ONap) and N-t-butyloxycarbonyl-L-alanine-p-nitrophenyl ester (Boc-Ala-ONp), but unlike the highly basic elastase isozymes, SE barely migrate into 13% acrylamide gels during cationic electrophoresis at pH 4.3. Hydrolysis of Ac-DL-Ala-1-ONap by SE requires the presence of Triton in the gel, and hydrolysis of Boc-Ala-ONp by the same enzyme(s) is also enhanced in the presence of the detergent. Triton is not required for these activities, in the case of the elastase isozymes. Diisopropylfluorophosphate (Dip-F) inactivates both SE and the elastase isozymes, whereas Ac-(Ala)2-Pro-AlaCH2Cl (a powerful inactivator of the leucocyte elastase isozymes at 10-4 M concentration) does not inactivate SE at the same concentration. Immunochemical studies revealed antigenic cross-reaction between the rapidly migrating leucocyte elastase isozymes and SE. Two preparations of leucocyte granules from nonleukemic bone marrow cells showed no activity of the rapidly migrating elastase isozymes, but did contain SE activity. SE may be a precursor or zymogen form of the elastase isozymes, present in immature cells and partly retained through later stages of development.
人类外周血白细胞颗粒含有四种特征明确的弹性蛋白酶同工酶以及一两种迁移速度较慢的类弹性蛋白酶酯酶（SE），其特征尚未完全明确。SE能够水解典型的弹性蛋白酶合成底物，如N - 乙酰 - dl - 丙氨酸 - α - 萘酯（Ac - DL - Ala - 1 - ONap）和N - t - 丁氧羰基 - L - 丙氨酸 - 对硝基苯酯（Boc - Ala - ONp），但与碱性很强的弹性蛋白酶同工酶不同，在pH 4.3的阳离子电泳过程中，SE几乎不会迁移到13%丙烯酰胺凝胶中。SE对Ac - DL - Ala - 1 - ONap的水解需要凝胶中存在 Triton，并且在洗涤剂存在的情况下，同一种酶对Boc - Ala - ONp的水解也会增强。对于弹性蛋白酶同工酶的这些活性而言，Triton并非必需。二异丙基氟磷酸酯（Dip - F）能使SE和弹性蛋白酶同工酶都失活，而Ac - (Ala)2 - Pro - AlaCH2Cl（在10 - 4 M浓度下是白细胞弹性蛋白酶同工酶的强效失活剂）在相同浓度下不会使SE失活。免疫化学研究揭示了快速迁移的白细胞弹性蛋白酶同工酶与SE之间的抗原交叉反应。来自非白血病骨髓细胞的两种白细胞颗粒制剂未显示快速迁移的弹性蛋白酶同工酶活性，但确实含有SE活性。SE可能是弹性蛋白酶同工酶的前体或酶原形式，存在于未成熟细胞中，并在发育后期部分保留。
