Galdston M, Klein N, Rothstein E
Am Rev Respir Dis. 1975 Nov;112(5):629-32. doi: 10.1164/arrd.1975.112.5.629.
Polymorphonuclear leukocyte lysosomal esterolytic activity on the synthetic substrate, t-butyloxycarbonyl-L-alanine p-nitrophenyl ester was observed to correlate well with polymorphonuclear leukocyte granule elastase activity measured on the natural substrate, elastin, bound to rhodamine. In addition, the effect of highly specific, irreversible chloromethyl ketone elastase inhibitors on leukocyte lysosomal elastase activity was similar, using t-butyloxycarbonyl-L-alanine p-nitrophenyl ester or elastin-rhodamine as substrate. Whether polymorphonuclear leukocyte lysosomal granules contain two different enzymes, a true elastase with esterase activity and a similar esterase without elastase activity, as found in the human pancreas, is, as yet, unknown. Both enzyme activities have been identified in isoenzymes of purified human polymorphonuclear leukocyte lysosomal elastase. The correlations observed between the two enzymes, if present in polymorphonuclear leukocytes, are sufficiently strong to use the esterase assay for clinical purposes.
观察到多形核白细胞对合成底物叔丁氧羰基-L-丙氨酸对硝基苯酯的溶酶体酯解活性与在结合若丹明的天然底物弹性蛋白上测得的多形核白细胞颗粒弹性蛋白酶活性密切相关。此外，使用叔丁氧羰基-L-丙氨酸对硝基苯酯或弹性蛋白-若丹明作为底物时，高度特异性、不可逆的氯甲基酮弹性蛋白酶抑制剂对白细胞溶酶体弹性蛋白酶活性的影响相似。多形核白细胞溶酶体颗粒是否像在人胰腺中那样含有两种不同的酶，一种具有酯酶活性的真正弹性蛋白酶和一种没有弹性蛋白酶活性的类似酯酶，目前尚不清楚。在纯化的人多形核白细胞溶酶体弹性蛋白酶的同工酶中已鉴定出这两种酶活性。如果多形核白细胞中存在这两种酶，观察到的它们之间的相关性足够强，足以将酯酶测定用于临床目的。
