Ivanov D V, Tyazhelova T V, Lemonnier L, Kononenko N, Pestova A A, Nikitin E A, Prevarskaya N, Skryma R, Panchin Y V, Yankovsky N K, Baranova A V
Vavilov Institute of General Genetics, 3 Gubkina Str., 119991 Moscow, Russia.
FEBS Lett. 2003 Mar 27;539(1-3):156-60. doi: 10.1016/s0014-5793(03)00211-4.
We report the primary characterization of a new gene KCNRG mapped at chromosome band 13q14.3. This gene includes three exons and has two alternatively spliced isoforms that are expressed in normal tissues and in some tumor cell lines. Protein KCNRG has high homology to tetramerization domain of voltage-gated K+ channels. Using the patch-clamp technique we determined that KCNRG suppresses K+ channel activity in human prostate cell line LNCaP. It is known that selective blockers of K+ channels suppress lymphocyte and LNCaP cell line proliferation. We suggest that KCNRG is a candidate for a B-cell chronic lymphocytic leukemia and prostate cancer tumor suppressor gene.
我们报告了一个定位在染色体13q14.3带的新基因KCNRG的初步特征。该基因包含三个外显子，有两种可变剪接异构体，在正常组织和一些肿瘤细胞系中表达。蛋白质KCNRG与电压门控钾通道的四聚化结构域具有高度同源性。利用膜片钳技术，我们确定KCNRG可抑制人前列腺癌细胞系LNCaP中的钾通道活性。已知钾通道的选择性阻滞剂可抑制淋巴细胞和LNCaP细胞系的增殖。我们认为KCNRG是B细胞慢性淋巴细胞白血病和前列腺癌肿瘤抑制基因的候选者。
