Hou Gui-rong, Zeng Kang, Zhou Zai-gao, Li Guo-feng
Department of Dermatology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2003 Mar;23(3):216-8.
To prepare two different liposome gels containing podophyllotoxin (PPT) and compare their pharmaceutical properties.
PPT-Dipalmitoylphosphatidylcholine (DPPC) liposome and PPT-soya lecithin liposome were prepared by reverse phase evaporation technique, and optical and electron microscopy were performed respectively for examining the appearances of the 2 liposomes and the diameter of the particles. Gel filtration was employed to assess the embedding ratio of the liposomes.
Under optical microscope, PPT-DPPC liposome was observed to be composed of spherical particles with diameter range of 2.05 to 0.825 microm (average 1.45 microm). The diameter range of the particles of PPT-soya lecithin liposome is 7.59 to 1.445 microm (average 3.78 microm). PPT-DPPC liposome appeared multivesicular under electron microscope, with the embedding ratio of 73.8%, and 79.1% for PPT-soya lecithin liposome (n=3). PPT-DPPC liposome had a embedding ratio of 65.2%, 58.8% and 56.4% after preserved 1, 3, 6 months respectively. As for PPT-soya lecithin liposome, the embedding ratios were 70.3%, 60.4%, and 51.3% respectively.
The PPT in the liposomes prepared by reverse phase evaporation technique is evenly distributed throughout the gel. The preparation is relatively simple with high embedding ratio.
制备两种含鬼臼毒素（PPT）的不同脂质体凝胶，并比较它们的药学性质。
采用逆相蒸发技术制备PPT-二棕榈酰磷脂酰胆碱（DPPC）脂质体和PPT-大豆卵磷脂脂质体，分别用光学显微镜和电子显微镜观察两种脂质体的外观及颗粒直径。采用凝胶过滤法评估脂质体的包封率。
光学显微镜下，观察到PPT-DPPC脂质体由直径范围为2.05至0.825微米（平均1.45微米）的球形颗粒组成。PPT-大豆卵磷脂脂质体颗粒的直径范围为7.59至1.445微米（平均3.78微米）。电子显微镜下，PPT-DPPC脂质体呈多囊泡状，包封率为73.8%，PPT-大豆卵磷脂脂质体的包封率为79.1%（n = 3）。PPT-DPPC脂质体在保存1、3、6个月后的包封率分别为65.2%、58.8%和56.4%。对于PPT-大豆卵磷脂脂质体，包封率分别为70.3%、60.4%和51.3%。
采用逆相蒸发技术制备的脂质体中的PPT均匀分布于整个凝胶中。该制剂制备相对简单，包封率高。
