Wang Hong-wei, Chen Zheng-liang, Zuo Da-ming, Lu Xiao, Yu Xin-pei
Department of Immunology, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2003 Mar;23(3):236-8, 241.
To obtain full-length cDNA encoding mouse mannan-binding lectin C(MBL-C) polypeptide.
The cDNA encoding mouse MBL-C was isolated from Balb/c mouse liver cells by reverse transcriptase (RT)-PCR and inserted into pUC-T vector, and the encoding region structure of the DNA was analyzed to understand its evolutionary relationship with single human MBL homologues.
The amplified mouse MBL-C cDNA, which was 735 bp in length, encoded 245 amino acid residues, and its structural analysis showed 100% homology with published mouse MBL-C gene sequence and 71.4% homology with MBL gene of Chinese human.
The gene encoding Balb/c mouse MBL-C has been successfully isolated, which may facilitate further study of the innate immune functions of MBL molecule in vivo.
获取编码小鼠甘露聚糖结合凝集素C（MBL-C）多肽的全长cDNA。
通过逆转录酶（RT）-PCR从Balb/c小鼠肝细胞中分离出编码小鼠MBL-C的cDNA，并将其插入pUC-T载体，分析该DNA的编码区结构，以了解其与人类单一MBL同源物的进化关系。
扩增得到的小鼠MBL-C cDNA长度为735 bp，编码245个氨基酸残基，其结构分析显示与已发表的小鼠MBL-C基因序列具有100%的同源性，与中国人的MBL基因具有71.4%的同源性。
已成功分离出编码Balb/c小鼠MBL-C的基因，这可能有助于进一步研究MBL分子在体内的固有免疫功能。
