Pagliari Carla, Sotto Mírian N
Departamento de Patologia, Faculdade de Medicina da Universidade de São Paulo, Brazil.
Am J Dermatopathol. 2003 Apr;25(2):107-12. doi: 10.1097/00000372-200304000-00003.
We demonstrated and quantified by immunohistochemistry epidermal Langerhans cells, CD34+ dermal dendrocytes (DDs), and cells expressing TNFalpha, interferon-gamma (IFNgamma), IL-5, and IL-10 in skin lesions of paracoccidioidomycosis (PCM). Sixty-one biopsies were classified in three groups according to the pattern of tissue response: Group 1, well-organized granuloma; Group 2, poorly organized granuloma; and Group 3, both kinds of granuloma. Langerhans cells had short and irregular dendrites in all groups and were decreased in number in Groups 1 and 2. CD34+ DDs did not differ in number from the control group. Group 1 was characterized by many cells expressing IFNgamma. Groups 2 and 3 exhibited large numbers of cells expressing IL-5 and IL-10. The data obtained suggest that well-organized granulomas reflect a better cellular immune response, and the large number of cells expressing IL-5 and IL-10 in Group 2 indicate an ineffective response in PCM skin lesions. Both kinds of granuloma in the same cutaneous lesion probably represent an intermediate response between the anergic and hyperergic poles. Group 3 also showed higher numbers of cells expressing TNFalpha when compared with the control group. Some cells expressing TNFalpha were dendritic and localized around the granuloma similar to the factor XIIIa+ DD localization that we previously described.
我们通过免疫组织化学方法对副球孢子菌病（PCM）皮肤病变中的表皮朗格汉斯细胞、CD34⁺真皮树突状细胞（DDs）以及表达肿瘤坏死因子α（TNFα）、干扰素γ（IFNγ）、白细胞介素-5（IL-5）和白细胞介素-10的细胞进行了鉴定和定量分析。根据组织反应模式，将61份活检标本分为三组：第1组，组织良好的肉芽肿；第2组，组织不良的肉芽肿；第3组，两种肉芽肿均有。在所有组中，朗格汉斯细胞的树突短且不规则，在第1组和第2组中数量减少。CD34⁺ DDs的数量与对照组无差异。第1组的特征是有许多表达IFNγ的细胞。第2组和第3组表现出大量表达IL-5和IL-10的细胞。所获得的数据表明，组织良好的肉芽肿反映了较好的细胞免疫反应，第2组中大量表达IL-5和IL-10的细胞表明PCM皮肤病变中的反应无效。同一皮肤病变中的两种肉芽肿可能代表了无反应性和高反应性两极之间的中间反应。与对照组相比，第3组中表达TNFα的细胞数量也更多。一些表达TNFα的细胞呈树突状，位于肉芽肿周围，类似于我们之前描述的因子ⅩⅢa⁺ DD的定位。
