Lin Jinxing, Jiang Wusheng, Liu Donghua C
Institute of Botany, Chinese Academy of Sciences, Xiangshan, Beijing 100093, China.
Bioresour Technol. 2003 Jan;86(2):151-5. doi: 10.1016/s0960-8524(02)00152-9.
The effects of different concentrations of copper sulfate on the growth of and the accumulation of Cu2+ by root, hypocotyl, cotyledon and leaf growth of sunflower (Helianthus annuus L.) were examined in this study. The concentrations of copper sulfate (CuSO4 x 5H2O) used were in the range from 10(-5) to 10(-3) M. Seedlings exposed to 10(-5) M Cu2+ solution exhibited a 33% increase in growth (P < 0.005) when compared with the root length of the control. The seedlings treated with 10(-3) M Cu2+ were significantly inhibited in shoot growth (P < 0.005). The Cu2+ content in roots, hypocotyls, cotyledons and leaves increased with increasing solution Cu2+ concentration. The roots of plants exposed to 10(-3) M Cu2+ accumulated a large amount of Cu (1070 microgram/g DW), and the Cu2+ level was approximately 25 fold higher than that of control. The Cu2+ contents in sunflower roots treated with 10(-4) and 10(-5) M Cu2+ were about 3.3 and 2.6 fold higher than the control, respectively. Also, the Cu2- level of the roots exposed to 10(-3) M Cu2+ was approximately 7.7 and 9.8 fold respectively, in comparison with the roots of plants grown in 10(-4) and 10(-5) M Cu2+. At 10(-3) M Cu2+, the Cu accumulated mainly in the roots (about 73%), and small amounts of Cu2+ (27%) were translocated to the hypocotyls, cotyledons and leaves. The Cu2+ concentration in the roots was less than that of the above parts of seedlings in treated groups with 10(-5) - 10(-4) M Cu2+. H. annuus has potential ability to accumulate Cu without being overly sensitive to Cu toxicity.
本研究考察了不同浓度硫酸铜对向日葵（Helianthus annuus L.）根系、下胚轴、子叶和叶片生长以及铜离子积累的影响。所用硫酸铜（CuSO₄·5H₂O）浓度范围为10⁻⁵至10⁻³M。与对照根系长度相比，暴露于10⁻⁵M Cu²⁺溶液中的幼苗生长增加了33%（P < 0.005）。用10⁻³M Cu²⁺处理的幼苗地上部生长受到显著抑制（P < 0.005）。根系、下胚轴、子叶和叶片中的铜离子含量随溶液中铜离子浓度的增加而增加。暴露于10⁻³M Cu²⁺的植物根系积累了大量的铜（1070微克/克干重），铜离子水平比对照高约25倍。用10⁻⁴和10⁻⁵M Cu²⁺处理的向日葵根系中的铜离子含量分别比对照高约3.3倍和2.6倍。此外，与生长在10⁻⁴和10⁻⁵M Cu²⁺中的植物根系相比，暴露于10⁻³M Cu²⁺的根系中的铜离子水平分别约高7.7倍和9.8倍。在10⁻³M Cu²⁺时，铜主要积累在根系中（约73%），少量铜离子（27%）转运到下胚轴、子叶和叶片中。在10⁻⁵ - 10⁻⁴M Cu²⁺处理组中，根系中的铜离子浓度低于幼苗上述部位。向日葵具有积累铜的潜在能力，且对铜毒性不过度敏感。
