Sinowatz F, Wessa E, Neumüller C, Palma G
Lehrstuhl für Tieranatomie II, University of Munich, Munich, Germany.
Reprod Domest Anim. 2003 Apr;38(2):141-6. doi: 10.1046/j.1439-0531.2003.00401.x.
Sperm binding and sperm penetration of the zona pellucida (zp) are regarded as species-specific. In this investigation, the interactions between bovine oocytes and porcine, respectively, equine spermatozoa have been studied under in vitro conditions and compared with the normal in vitro fertilization of bovine oocytes by bovine sperm. Surprisingly, many of the heterologous spermatozoa adhered firmly to the bovine oocytes and could not be removed by intense washing. On average, more than 100 boar or equine spermatozoa were bound to the zp of bovine oocytes. Electron microscopic studies clearly demonstrated that porcine sperm attached to the zona and underwent the acrosome reaction. Equine spermatozoa displayed a similar binding affinity, but unlike the porcine spermatozoa even penetrated the zp and were taken up into the oocyte after a longer period of co-incubation. Considering these new results the dogma of a strict species specificity of sperm zona interactions under in vitro conditions has to be reconsidered.
精子与透明带（ZP）的结合及穿透被认为具有物种特异性。在本研究中，分别对牛卵母细胞与猪、马精子之间的相互作用进行了体外条件下的研究，并与牛精子对牛卵母细胞的正常体外受精进行了比较。令人惊讶的是，许多异种精子牢固地附着在牛卵母细胞上，剧烈冲洗也无法将其去除。平均而言，超过100个公猪或马的精子附着在牛卵母细胞的透明带上。电子显微镜研究清楚地表明，猪精子附着在透明带上并发生顶体反应。马精子表现出类似的结合亲和力，但与猪精子不同的是，经过较长时间的共同孵育后，马精子甚至穿透了透明带并被卵母细胞摄取。考虑到这些新结果，体外条件下精子与透明带相互作用严格的物种特异性这一教条必须重新审视。
