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精子糖结合蛋白介导的精子与透明带的结合在猪和牛之间的体外不是种属特异性的。

Binding of Sperm to the Zona Pellucida Mediated by Sperm Carbohydrate-Binding Proteins is not Species-Specific in Vitro between Pigs and Cattle.

机构信息

Graduate School of Science, Chiba University, Chiba 263-8522, Japan.

National Institute of Agrobiological Sciences, Ibaraki 305-8602, Japan.

出版信息

Biomolecules. 2013 Jan 25;3(1):85-107. doi: 10.3390/biom3010085.

DOI:10.3390/biom3010085
PMID:24970158
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4030887/
Abstract

Carbohydrates are candidates for the basis of species-selective interaction of gametes during mammalian fertilization. In this study, we sought to clarify the roles of sugar residues in the species-selective, sperm-oocyte interaction in pigs and cattle. Acrosome-intact porcine and bovine sperm exhibited their strongest binding affinities for β-Gal and α-Man residues, respectively. Porcine-sperm specificity changed from β-Gal to α-Man after the acrosome reaction, while bovine-sperm specificity did not. Binding of acrosome-intact and acrosome-reacted sperm decreased after trypsinization, indicating that the carbohydrate-binding components are proteins. While immature oocytes bound homologous sperm preferentially to heterologous sperm, oocytes matured in vitro bound similar numbers of homologous and heterologous sperm. Lectin staining revealed the aggregation of α-Man residues on the outer surface of the porcine zona during maturation. In both species, zona-free, mature oocytes bound homologous sperm preferentially to heterologous sperm. The lectin-staining patterns of the zona pellucida and zona-free oocytes coincided with the carbohydrate-binding specificities of acrosome-intact and acrosome-reacted sperm, respectively, supporting the involvement of carbohydrates in gamete recognition in pigs and cattle. These results also indicate that sperm-zona pellucida and sperm-oolemma bindings are not strictly species-specific in pigs and cattle, and further suggest that sperm penetration into the zona and/or fusion with oolemma may be species-specific between pigs and cattle.

摘要

碳水化合物是哺乳动物受精过程中配子种间选择性相互作用的候选物质。在这项研究中，我们试图阐明糖残基在猪和牛种间精子-卵相互作用中的作用。顶体完整的猪和牛精子分别对β-Gal 和α-Man 残基表现出最强的结合亲和力。顶体反应后，猪精子的特异性从β-Gal 转变为α-Man，而牛精子的特异性没有改变。顶体完整和顶体反应后的精子的结合在胰蛋白酶消化后减少，表明碳水化合物结合成分是蛋白质。虽然未成熟卵与同源精子优先结合，而体外成熟的卵与同源和异源精子结合的数量相似。凝集素染色显示猪卵透明带在成熟过程中α-Man 残基在其外表面聚集。在这两种物种中，无透明带、成熟的卵与同源精子优先结合，而与异源精子结合较少。透明带和无透明带卵的凝集素染色模式分别与顶体完整和顶体反应精子的碳水化合物结合特异性一致，表明碳水化合物参与了猪和牛的配子识别。这些结果还表明，猪和牛的精子-透明带和精子-卵膜的结合并不是严格的种间特异性的，进一步表明精子穿透透明带和/或与卵膜融合在猪和牛之间可能是种间特异性的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1beb/4030887/23135b673b4a/biomolecules-03-00085-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1beb/4030887/2c594fe63098/biomolecules-03-00085-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1beb/4030887/f224d8cdf2d0/biomolecules-03-00085-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1beb/4030887/4b479a54babf/biomolecules-03-00085-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1beb/4030887/5cb12c10e1d7/biomolecules-03-00085-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1beb/4030887/b9e4062603e3/biomolecules-03-00085-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1beb/4030887/29694e2807f7/biomolecules-03-00085-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1beb/4030887/7ee44a822077/biomolecules-03-00085-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1beb/4030887/23135b673b4a/biomolecules-03-00085-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1beb/4030887/2c594fe63098/biomolecules-03-00085-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1beb/4030887/f224d8cdf2d0/biomolecules-03-00085-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1beb/4030887/4b479a54babf/biomolecules-03-00085-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1beb/4030887/5cb12c10e1d7/biomolecules-03-00085-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1beb/4030887/b9e4062603e3/biomolecules-03-00085-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1beb/4030887/29694e2807f7/biomolecules-03-00085-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1beb/4030887/7ee44a822077/biomolecules-03-00085-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1beb/4030887/23135b673b4a/biomolecules-03-00085-g008.jpg

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