Pinheiro E T, Gomes B P F A, Ferraz C C R, Teixeira F B, Zaia A A, Souza Filho F J
Endodontic Area, Dental School of Piracicaba, University of Campinas, Piracicaba, SP, Brazil.
Oral Microbiol Immunol. 2003 Apr;18(2):100-3. doi: 10.1034/j.1399-302x.2003.00058.x.
Studies of the microbiota from the canals of teeth with failure of endodontic therapy have revealed that it differs markedly from that of untreated necrotic dental pulps. This study aimed to evaluate the microbiota of 30 root-filled teeth with persisting periapical lesions and to test the antibiotic susceptibility of the most prevalent species. Microbial samples, isolation and speciation were done using advanced microbiologic techniques for anaerobic species. A total of 55 bacterial species were isolated, 80% were gram-positives and 58% facultative anaerobic microorganisms. The bacterial genera most frequently recovered were Enterococcus, Streptococcus, Peptostreptococcus and Actinomyces. Antibiotic sensitivity of Enterococcus faecalis and Peptostreptococcus spp. was accomplished with the E-test system. All species studied were susceptible to benzylpenicillin, amoxicillin, amoxicillin combined with clavulanate. However, 20% of the E.faecalis strains were resistant to erythromycin and 60% to azithromycin. It was concluded that microbial flora in canals after endodontic failure comprised predominantly facultative anaerobes and gram-positive species. E.faecalis was the species most frequently isolated and showed erythromycin and azithromycin resistance among the isolates.
对根管治疗失败的患牙根管内微生物群的研究表明，其与未经治疗的坏死牙髓的微生物群有显著差异。本研究旨在评估30颗有根尖周病变的根管充填牙的微生物群，并检测最常见菌种的抗生素敏感性。使用先进的厌氧菌微生物技术进行微生物样本的采集、分离和鉴定。共分离出55种细菌，80%为革兰氏阳性菌，58%为兼性厌氧微生物。最常分离出的细菌属为肠球菌、链球菌、消化链球菌和放线菌。使用E-test系统检测粪肠球菌和消化链球菌属的抗生素敏感性。所有研究的菌种对苄青霉素、阿莫西林、阿莫西林联合克拉维酸均敏感。然而，20%的粪肠球菌菌株对红霉素耐药，60%对阿奇霉素耐药。得出的结论是，根管治疗失败后根管内的微生物群主要由兼性厌氧菌和革兰氏阳性菌组成。粪肠球菌是最常分离出的菌种，在所分离出的菌种中表现出对红霉素和阿奇霉素耐药。
