Hughes R C, Thurman P F, Stokes E
Z Immunitatsforsch Exp Klin Immunol. 1975 Jul;149(2-4):126-35.
The maximum porosity of Bacillus subtilis and Bacillus licheniformis cell walls was estimated by two independent and relatively simple methods. Peptidoglycan was isolated from Bacillus subtilis cell wall preparations and used as an insoluble support for exclusion chromatography of dextrans of known average molecular size. In an alternative approach the leakage of radioactively labelled proteins from Bacillus licheniformis cells incubated in butanol-saline mixtures was measured and their size estimated by exclusion chromatography on Sephadex G-100. Under these conditions the permeability barrier of the cytoplasmic membrane was destroyed with preservation of the structural integrity of the outer cell wall. The apparent exclusion threshold of the cell wall of either organism as determined by these means corresponded to molecules with a diffusional radius of not more than 2.5 nm.
通过两种独立且相对简单的方法估算了枯草芽孢杆菌和地衣芽孢杆菌细胞壁的最大孔隙率。从枯草芽孢杆菌细胞壁制剂中分离出肽聚糖，并将其用作已知平均分子大小的葡聚糖排阻色谱的不溶性支持物。在另一种方法中，测量了在丁醇 - 盐混合物中孵育的地衣芽孢杆菌细胞中放射性标记蛋白质的泄漏情况，并通过在葡聚糖G - 100上的排阻色谱法估算其大小。在这些条件下，细胞质膜的渗透屏障被破坏，而外细胞壁的结构完整性得以保留。通过这些方法确定的任何一种生物体细胞壁的表观排阻阈值对应于扩散半径不超过2.5 nm的分子。
