Wyrick P B, Rogers H J
J Bacteriol. 1973 Oct;116(1):456-65. doi: 10.1128/jb.116.1.456-465.1973.
Quantitative mass conversion of intact bacterial cells of Bacillus subtilis and B. licheniformis to L-phase variants has been effected after lysozyme treatment. After subculture of the unstable L-phase variants for several months in the presence of methicillin, stable L-phase variants were obtained which grew in the absence of the antibiotic and were then unable to revert to the classical bacterial phase under conditions which gave rise to mass reversion of the protoplasts and unstable L-variants. These stable L-phase variants, which retained many of the physiological properties of the bacterium from which they were derived, were capable of growing exponentially and multiplying in liquid medium. Their morphology and apparent modes of reproduction were consistent with that described for other L-phase variants. The morphological events, as monitored by the electron microscope, of the reversion to the intact bacterial phase of an unstable L-phase variant of B. licheniformis are described.
经溶菌酶处理后，已实现枯草芽孢杆菌和地衣芽孢杆菌完整细菌细胞向L型变体的定量质量转化。在甲氧西林存在的情况下，将不稳定的L型变体传代培养数月后，获得了稳定的L型变体，这些变体在没有抗生素的情况下生长，然后在导致原生质体和不稳定L变体大量回复的条件下无法回复到经典细菌相。这些稳定的L型变体保留了其来源细菌的许多生理特性，能够在液体培养基中呈指数生长和繁殖。它们的形态和明显的繁殖方式与其他L型变体所描述的一致。描述了用地衣芽孢杆菌不稳定L型变体回复到完整细菌相的形态学事件，该事件通过电子显微镜监测。
