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本文引用的文献

1
Evolutionary relationship of Alw26I, Eco31I and Esp3I, restriction endonucleases that recognise overlapping sequences.识别重叠序列的限制性内切酶Alw26I、Eco31I和Esp3I的进化关系。
Mol Genet Genomics. 2002 Jul;267(5):664-72. doi: 10.1007/s00438-002-0701-6. Epub 2002 Jun 19.
2
The 3' 5' exonucleases.3'→5'核酸外切酶
Nat Rev Mol Cell Biol. 2002 May;3(5):364-76. doi: 10.1038/nrm804.
3
Evolutionary relationship between different subgroups of restriction endonucleases.限制性内切酶不同亚组之间的进化关系。
J Biol Chem. 2002 Apr 19;277(16):14306-14. doi: 10.1074/jbc.M111625200. Epub 2002 Feb 4.
4
Structure of Ocr from bacteriophage T7, a protein that mimics B-form DNA.来自噬菌体T7的Ocr结构，一种模拟B型DNA的蛋白质。
Mol Cell. 2002 Jan;9(1):187-94. doi: 10.1016/s1097-2765(02)00435-5.
5
2001 Fred Griffith review lecture. Immigration control of DNA in bacteria: self versus non-self.2001年弗雷德·格里菲斯回顾讲座。细菌中DNA的免疫控制：自我与非自我。
Microbiology (Reading). 2002 Jan;148(Pt 1):3-20. doi: 10.1099/00221287-148-1-3.
6
The type IIs restriction endonuclease BspMI is a tetramer that acts concertedly at two copies of an asymmetric DNA sequence.IIs型限制性内切酶BspMI是一种四聚体，它在一个不对称DNA序列的两个拷贝上协同作用。
J Biol Chem. 2002 Feb 8;277(6):4034-41. doi: 10.1074/jbc.M108442200. Epub 2001 Nov 29.
7
Characterization of AloI, a restriction-modification system of a new type.新型限制修饰系统AloI的特性分析
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8
DNA cleavage by type III restriction-modification enzyme EcoP15I is independent of spacer distance between two head to head oriented recognition sites.III型限制修饰酶EcoP15I的DNA切割与两个头对头定向识别位点之间的间隔距离无关。
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9
Nucleoside triphosphate-dependent restriction enzymes.核苷三磷酸依赖性限制酶
Nucleic Acids Res. 2001 Sep 15;29(18):3728-41. doi: 10.1093/nar/29.18.3728.
10
Structure and function of type II restriction endonucleases.II型限制性核酸内切酶的结构与功能。
Nucleic Acids Res. 2001 Sep 15;29(18):3705-27. doi: 10.1093/nar/29.18.3705.

核酸外切酶与核酸内切酶之间的功能协作——限制酶进化的基础

Functional cooperation between exonucleases and endonucleases--basis for the evolution of restriction enzymes.

作者信息

Raghavendra Nidhanapathi K, Rao Desirazu N

机构信息

Department of Biochemistry, Indian Institute of Science, Bangalore-560012, India.

出版信息

Nucleic Acids Res. 2003 Apr 1;31(7):1888-96. doi: 10.1093/nar/gkg275.

DOI:10.1093/nar/gkg275
PMID:12655005
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC152791/
Abstract

Many types of restriction enzymes cleave DNA away from their recognition site. Using the type III restriction enzyme, EcoP15I, which cleaves DNA 25-27 bp away from its recognition site, we provide evidence to show that an intact recognition site on the cleaved DNA sequesters the restriction enzyme and decreases the effective concentration of the enzyme. EcoP15I restriction enzyme is shown here to perform only a single round of DNA cleavage. Significantly, we show that an exonuclease activity is essential for EcoP15I restriction enzyme to perform multiple rounds of DNA cleavage. This observation may hold true for all restriction enzymes cleaving DNA sufficiently far away from their recognition site. Our results highlight the importance of functional cooperation in the modulation of enzyme activity. Based on results presented here and other data on well-characterised restriction enzymes, a functional evolutionary hierarchy of restriction enzymes is discussed.

摘要

许多类型的限制酶在远离其识别位点的地方切割DNA。使用III型限制酶EcoP15I，它在远离其识别位点25 - 27个碱基对处切割DNA，我们提供证据表明，切割后的DNA上完整的识别位点会隔离限制酶并降低酶的有效浓度。此处显示EcoP15I限制酶仅进行一轮DNA切割。值得注意的是，我们表明核酸外切酶活性对于EcoP15I限制酶进行多轮DNA切割至关重要。这一观察结果可能适用于所有在远离其识别位点处切割DNA的限制酶。我们的结果突出了功能协作在调节酶活性中的重要性。基于此处呈现的结果以及关于特征明确的限制酶的其他数据，讨论了限制酶的功能进化层次结构。