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S-腺苷同型半胱氨酸和DNA末端刺激III型限制性内切酶EcoPI中的混杂核酸酶活性。

S-adenosyl homocysteine and DNA ends stimulate promiscuous nuclease activities in the Type III restriction endonuclease EcoPI.

作者信息

Peakman Luke J, Szczelkun Mark D

机构信息

DNA-Protein Interactions Unit, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK.

出版信息

Nucleic Acids Res. 2009 Jul;37(12):3934-45. doi: 10.1093/nar/gkp267. Epub 2009 Apr 28.

DOI:10.1093/nar/gkp267
PMID:19401438
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2709564/
Abstract

In the absence of the methyl donor S-adenosyl methionine and under certain permissive reaction conditions, EcoPI shows non-specific endonuclease activity. We show here that the cofactor analogue S-adenosyl homocysteine promotes this promiscuous DNA cleavage. Additionally, an extensive exonuclease-like processing of the DNA is also observed that can even result in digestion of non-specific DNA in trans. We suggest a model for how DNA communication events initiating from non-specific sites, and in particular free DNA ends, could produce the observed cleavage patterns.

摘要

在缺乏甲基供体S-腺苷甲硫氨酸且在某些允许的反应条件下，EcoPI表现出非特异性核酸内切酶活性。我们在此表明，辅因子类似物S-腺苷高半胱氨酸促进了这种杂乱的DNA切割。此外，还观察到对DNA进行广泛的类似核酸外切酶的加工，甚至可导致反式非特异性DNA的消化。我们提出了一个模型，说明从非特异性位点（特别是游离DNA末端）起始的DNA通讯事件如何产生观察到的切割模式。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/c83ec8f17752/gkp267f11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/9375a22eabf5/gkp267f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/19360772ffa3/gkp267f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/a8033a37274e/gkp267f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/7500efc5c01b/gkp267f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/8941d7047b73/gkp267f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/969de79fb448/gkp267f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/5e48441b594c/gkp267f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/177e1da139ce/gkp267f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/d831798f4751/gkp267f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/e82e34e6ac35/gkp267f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/c83ec8f17752/gkp267f11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/9375a22eabf5/gkp267f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/19360772ffa3/gkp267f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/a8033a37274e/gkp267f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/7500efc5c01b/gkp267f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/8941d7047b73/gkp267f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/969de79fb448/gkp267f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/5e48441b594c/gkp267f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/177e1da139ce/gkp267f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/d831798f4751/gkp267f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/e82e34e6ac35/gkp267f10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/087a/2709564/c83ec8f17752/gkp267f11.jpg

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