La Scola Bernard, Mallet Marie-Noëlle, Grimont Patrick A D, Raoult Didier
Unité des Rickettsies, CNRS UPRESA 6020, Faculté de Médecine, Université de la Méditerrannée, 27 Boulevard Jean Moulin, 13385 Marseille Cedex 05, France.
Unité des Entérobactéries, Unité INSERM 199, Institut Pasteur, 75724, Paris Cedex 15, France.
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):15-20. doi: 10.1099/ijs.0.02127-0.
On the basis of phenotypic and DNA relatedness data, three novel species of the genus Bosea are proposed, Bosea massiliensis (63287T =CIP 106336T =CCUG 43117T), Bosea vestrisii (34635T =CIP 106340T =CCUG 43114T) and Bosea eneae (34614T =CIP 106338T =CCUG 43111T). The original description of the genus Bosea included thiosulphate oxidation as a phenotypic feature, when the sole and type species of the genus, Bosea thiooxidans, was proposed. The three novel species described herein were not able to oxidize thiosulphate; thus, it is proposed that this characteristic be removed from the description of the genus and considered as specific for B. thiooxidans. The novel species of the genus Bosea proposed here form a well-separated cluster in the Bradyrhizobium group of the alpha-2 subclass of the Proteobacteria, on the basis of 16S rDNA gene sequence analysis. However, 16S rDNA gene sequence analysis was not sufficient to delineate the species; hence, DNA-DNA relatedness and phenotypic data were also required. All of the novel species described in this study are fastidious bacteria isolated from a hospital water supply, using co-cultivation with amoebae. This group of bacteria are hypothesized to be a potential cause of nosocomial infections. For treatment of infections caused by these novel bacteria, doxycycline appears to be the sole antibacterial compound with a consistently low MIC value.
基于表型和DNA相关性数据，提出了博斯氏菌属的三个新物种，即马赛博斯氏菌（63287T =CIP 106336T =CCUG 43117T）、韦氏博斯氏菌（34635T =CIP 106340T =CCUG 43114T）和埃内氏博斯氏菌（34614T =CIP 106338T =CCUG 43111T）。博斯氏菌属的原始描述将硫代硫酸盐氧化作为一种表型特征，当时该属的唯一模式种硫氧化博斯氏菌被提出。本文描述的这三个新物种不能氧化硫代硫酸盐；因此，建议从该属的描述中删除这一特征，并认为这是硫氧化博斯氏菌特有的特征。基于16S rDNA基因序列分析，这里提出的博斯氏菌属新物种在变形菌门α-2亚类的慢生根瘤菌组中形成了一个界限分明的聚类。然而，16S rDNA基因序列分析不足以区分这些物种；因此，还需要DNA-DNA相关性和表型数据。本研究中描述的所有新物种都是从医院供水系统中分离出的苛求菌，采用与变形虫共培养的方法获得。这组细菌被认为是医院感染的潜在病因。对于由这些新细菌引起的感染，强力霉素似乎是唯一一种MIC值始终较低的抗菌化合物。
