Department of Safety and Quality of Fruit and Vegetables, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, Haid-und-Neu-Straße 9, 76131, Karlsruhe, Germany.
Department of Safety and Quality of Meat, Max Rubner-Institut, Federal Research Institute of Nutrition and Food, E.-C.-Baumann-Straße 20, 95326, Kulmbach, Germany.
Curr Microbiol. 2024 Jun 5;81(7):212. doi: 10.1007/s00284-024-03717-6.
Strain ZW T0_25 was isolated from an onion sample (Allium cepa var. Hytech F1) within a storage trial and proofed to be a novel, aerobic, Gram-stain negative, rod-shaped bacterial strain. Analyses of the 16S rRNA gene sequence and of the whole draft genome sequences, i.e., digital DNA-DNA hybridization (dDDH), Average Nucleotide Identity (ANI) and Average Amino Acid Identity (AAI) showed that this strain represents a new species of the genus Bosea. The genome size of strain ZW T0_25 is 6.19 Mbp, and the GC content is 66.9%. As whole cell sugars, rhamnose, ribose and glucose were identified. Ubiquinone Q-10 is the major respiratory quinone with 97.8%. Polar lipids in strain ZW T0_25 are composed of one phosphatidylethanolamine, one phosphatidylglycerol, one aminophospholipid, two aminolipids, one glycolipid and two phospholipids whereas the fatty acid profile predominantly consists of C18:1 w7c (63.3%), C16:1 w7c (19.5%) and C16:0 (7.1%). Phenotypic traits were tested in the wet lab as well as predicted in silico from genome data. Therefore, according to this polyphasic approach, the new name Bosea rubneri sp. nov. with the type strain ZW T0_25 (= DSM 116094 = LMG 33093) is proposed.
菌株 ZW T0_25 从洋葱样本（Allium cepa var. Hytech F1）中分离得到，在贮藏试验中证明是一种新型需氧革兰氏阴性杆状细菌。16S rRNA 基因序列和全基因组草图序列分析，即数字 DNA-DNA 杂交（dDDH）、平均核苷酸同一性（ANI）和平均氨基酸同一性（AAI）表明，该菌株代表 Bosea 属的一个新种。菌株 ZW T0_25 的基因组大小为 6.19 Mbp，GC 含量为 66.9%。作为全细胞糖，鉴定出鼠李糖、核糖和葡萄糖。泛醌 Q-10 是主要的呼吸醌，占 97.8%。菌株 ZW T0_25 的极性脂由一个磷脂酰乙醇胺、一个磷脂酰甘油、一个氨基磷脂、两个氨基脂、一个糖脂和两个磷脂组成，而脂肪酸图谱主要由 C18:1 w7c（63.3%）、C16:1 w7c（19.5%）和 C16:0（7.1%）组成。表型特征在湿实验室中进行了测试，并根据基因组数据进行了计算机预测。因此，根据这种多相方法，提出了一种新的 Bosea rubneri sp. nov.，其模式菌株为 ZW T0_25（=DSM 116094=LMG 33093）。
