Jin Yan, Kim Yeon-Ju, Hoang Van-An, Young Jung Sun, Nguyen Ngoc-Lan, Woo Min Jin, Wang Chao, Yang Deok-Chun
Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University.
J Gen Appl Microbiol. 2014;60(2):59-64. doi: 10.2323/jgam.60.59.
The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DCY69(T) is JX233806. A Gram-reaction-negative, oxidase- and catalase-positive, non-gliding motile strain, designated strain DCY69(T), was isolated from the soil of a ginseng field in the Republic of Korea. Colonies of strain DCY69(T) were circular, 0.5-1.5 mm diameter, yellow, and convex on an R2A agar plate after 2 days. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DCY69(T) belonged to the genus Flavobacterium with 90.5-98.3% gene sequence similarity. The major predominant quinone was MK-6. The major cellular fatty acids were iso-C15:0, iso-C17:0 3-OH, iso-C15:0 3-OH and summed feature 3 (containing C16:1ω7c and/or C16:1ω6c). The major polar lipids were phosphatidylethanolamine, one unidentified aminolipid and unidentified polar lipids (L1, L2). The genomic DNA G+C content of strain DCY69(T) was 35.0mol%. The strain DCY69(T) transformed ginsenoside Rb1 into Rd and F2. Based on the polyphasic taxonomic data, strain DCY69(T) is considered to represent a novel species of the genus Flavobacterium, for which the name Flavobacterium panaciterrae sp. nov. is proposed. The type strain is DCY69(T)(= KCTC 32392(T) = JCM 19161(T)), isolated from the soil of a ginseng field in the Republic of Korea.
菌株DCY69(T)的16S rRNA基因序列的GenBank/EMBL/DDBJ登录号为JX233806。从韩国人参田土壤中分离出一株革兰氏阴性、氧化酶和过氧化氢酶阳性、不滑动的运动菌株，命名为菌株DCY69(T)。菌株DCY69(T)的菌落呈圆形，直径0.5 - 1.5毫米，黄色，在R2A琼脂平板上培养2天后呈凸起状。基于16S rRNA基因序列的系统发育分析表明，菌株DCY69(T)属于黄杆菌属，基因序列相似性为90.5 - 98.3%。主要优势醌为MK - 6。主要细胞脂肪酸为异C15:0、异C17:0 3 - OH、异C15:0 3 - OH和特征性总和3（含C16:1ω7c和/或C16:1ω6c）。主要极性脂为磷脂酰乙醇胺、一种未鉴定的氨基脂和未鉴定的极性脂（L1、L2）。菌株DCY69(T)的基因组DNA G + C含量为35.0mol%。菌株DCY69(T)将人参皂苷Rb1转化为人参皂苷Rd和F2。基于多相分类学数据，菌株DCY69(T)被认为代表黄杆菌属的一个新物种，为此提出黄杆菌人参土栖菌新种（Flavobacterium panaciterrae sp. nov.）。模式菌株为DCY69(T)(= KCTC 32392(T) = JCM 19161(T))，从韩国人参田土壤中分离得到。
